Research Paper

The genus Phaeanthus (Annonaceae, Miliuseae) in Thailand: P. piyae sp. nov. and resurrection of P. lucidus, with molecular phylogenetic analyses

Chattida Wiya, Kithisak Aongyong, Anissara Damthongdee, Abdulromea Baka, Tanawat Chaowasku

Published on: 08 November 2021

Page: 509 - 516

DOI: 10.6165/tai.2021.66.509

  • BibTeX
    •
  • EndNote
    •
  • RIS
    •

    Abstract

    Phaeanthus piyae, a new species from southern Thailand, is described and illustrated. It is morphologically most similar to P. intermedius, which occurs in Peninsular Malaysia, Singapore, Riau Islands, and Sumatra. The new species can be distinguished from P. intermedius by the longer peduncles and pedicels; triangular (vs. filiform-narrowly triangular) sepals; and stigmas that are larger and ± elongated club-shaped (vs. ± ellipsoid). The conservation status of P. piyae is provisionally assessed. In addition, molecular phylogenetic analyses using up to seven plastid DNA regions (matK, ndhF, rbcL and ycf1 exons; trnL intron; psbA-trnH and trnL-trnF intergenic spacers) and including, among others, five Phaeanthus accessions are carried out. Phaeanthus piyae is sister to a clade composed of the remaining accessions of Phaeanthus. Furthermore, southern Thailand also harbors a second species of Phaeanthus, which appears to be P. lucidus, a heterotypic synonym of P. splendens. Phaeanthus lucidus is herein reinstated; it differs from the sister species P. splendens by possessing a lower number of stamens and carpels per flower, as well as dissimilar placentation. A key to the two species of Phaeanthus in Thailand is given.

    Keyword: Miliuseae, molecular phylogeny, new species, Phaeanthus, resurrection, taxonomy, Thailand

    Literature Cited

    Akaike, H. 1974. A new look at the statistical model identi?cation. IEEE Trans. Automat. Contr. 19(6): 716–723.
    DOI: 10.1109/TAC.1974.1100705View Article Google Scholar

    Chalermglin, P. 2001. Family Annonaceae. Baan Lae Suan, Bangkok, 368 pp. [In Thai].

    Chaowasku, T. 2020. Toward a phylogenetic reclassification of the subfamily Ambavioideae (Annonaceae): establishment of a new subfamily and a new tribe. Acta Bot. Bras. 34(3): 522–529.
    DOI: 10.1590/0102-33062020abb0051View Article Google Scholar

    Chaowasku, T., K. Aongyong, A. Damthongdee, H. Jongsook and D.M. Johnson. 2020. Generic status of Winitia (Annonaceae, Miliuseae) reaffirmed by molecular phylogenetic analysis, including a new species and a new combination from Thailand. Eur. J. Taxon. 659: 1–23.
    DOI: 10.5852/ejt.2020.659View Article Google Scholar

    Chaowasku, T., A. Damthongdee, H. Jongsook, D.T. Ngo, H.T. Le, D.M. Tran and S. Suddee. 2018a. Enlarging the monotypic Monocarpieae (Annonaceae, Malmeoideae): recognition of a second genus from Vietnam informed by morphology and molecular phylogenetics. Candollea 73(2): 261–275.
    DOI: 10.15553/c2018v732a11View Article Google Scholar

    Chaowasku, T., A. Damthongdee, H. Jongsook, M.S. Nuraliev, D.T. Ngo, H.T. Le, P. Lithanatudom, M. Osathanunkul, T. Deroin, B. Xue and J. Wipasa. 2018b. Genus Huberantha (Annonaceae) revisited: erection of Polyalthiopsis, a new genus for H. floribunda, with a new combination H. luensis. Ann. Bot. Fenn. 55(1–3): 121–136.
    DOI: 10.5735/085.055.0114View Article Google Scholar

    Chaowasku, T. and P.J.A. Ke?ler. 2006. Miliusa lanceolata (Annonaceae), a new species from Papua New Guinea. Blumea 51(3): 553–557.
    DOI: 10.3767/000651906X622102View Article Google Scholar

    Chaowasku, T., P.J.A. Ke?ler and L.W. Chatrou. 2013. Phylogeny of Miliusa (Magnoliales: Annonaceae: Malmeoideae: Miliuseae), with descriptions of two new species from Malesia. Eur. J. Taxon. 54: 1–21.
    DOI: 10.5852/ejt.2013.54View Article Google Scholar

    Chaowasku, T., D.C. Thomas, R.W.J.M. van der Ham, E.F. Smets, J.B. Mols and L.W. Chatrou. 2014. A plastid DNA phylogeny of tribe Miliuseae: insights into relationships and character evolution in one of the most recalcitrant major clades of Annonaceae. Amer. J. Bot. 101(4): 691–709.
    DOI: 10.3732/ajb.1300403View Article Google Scholar

    Chatrou, L.W., M.D. Pirie, R.H.J. Erkens, T.L.P. Couvreur, K.M. Neubig, J.R. Abbott, J.B. Mols, J.W. Maas, R.M.K. Saunders and M.W. Chase. 2012. A new subfamilial and tribal classification of the pantropical flowering plant family Annonaceae informed by molecular phylogenetics. Bot. J. Linn. Soc. 169(1): 5–40.
    DOI: 10.1111/j.1095-8339.2012.01235.xView Article Google Scholar

    Chatrou, L.W., I.M. Turner, B.B. Klitgaard, P.J.M. Maas and T.M.A. Utteridge. 2018. A linear sequence to facilitate curation of herbarium specimens of Annonaceae. Kew Bulletin 73(3): 1–10.
    DOI: 10.1007/s12225-018-9764-3View Article Google Scholar

    Chernomor, O., A. von Haeseler and B.Q. Minh. 2016. Terrace aware data structure for phylogenomic inference from supermatrices. Syst. Biol. 65(6): 997–1008.
    DOI: 10.1093/sysbio/syw037View Article Google Scholar

    Damthongdee, A., K. Aongyong and T. Chaowasku. 2021. Orophea sichaikhanii (Annonaceae), a new species from southern Thailand, with a key to the species of Orophea in Thailand and notes on some species. Plant Ecol. Evol. 154(2): 307–315.
    DOI: 10.5091/plecevo.2021.1780View Article Google Scholar

    Edgar, R.C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32(5): 1792–1797.
    DOI: 10.1093/nar/gkh340View Article Google Scholar

    Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4): 783–791.
    DOI: 10.2307/2408678View Article Google Scholar

    Gardner, S., P. Sidisunthorn and K. Chayamarit. 2015. Forest trees of southern Thailand, vol. 1: Acanthaceae to Escalloniaceae. Kobfai Printing Project, Bangkok, 748 pp.

    Ghogue, J.-P., B. Sonk? and T.L.P. Couvreur. 2017. Taxonomic revision of the African genera Brieya and Piptostigma (Annonaceae). Plant Ecol. Evol. 150(2): 173–216.
    DOI: 10.5091/plecevo.2017.1137View Article Google Scholar

    Guo, X., C.C. Tang, D.C. Thomas, T.L.P. Couvreur and R.M.K. Saunders. 2017. A mega-phylogeny of the Annonaceae: taxonomic placement of five enigmatic genera and recognition of a new tribe, Phoenicantheae. Sci. Rep. 7(1): 1–11.
    DOI: 10.1038/s41598-017-07252-2View Article Google Scholar

    Guo, X., J. Wang, B. Xue, D.C. Thomas, Y.C.F. Su, Y.H. Tan and R.M.K. Saunders. 2014. Reassessing the taxonomic status of two enigmatic Desmos species (Annonaceae): morphological and molecular phylogenetic support for a new genus, Wangia. J. Syst. Evol. 52(1): 1–15.
    DOI: 10.1111/jse.12064View Article Google Scholar

    Hasegawa, M., H. Kishino and T. Yano. 1985. Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J. Mol. Evol. 22(2): 160–174.
    DOI: 10.1007/BF02101694View Article Google Scholar

    Hewson, H.J. 1988. Plant indumentum. A handbook of terminology. Australian Government Publishing Service, Canberra, 27 pp.

    IUCN. 2012. IUCN Red List categories and criteria, version 3.1, 2nd ed. IUCN, Gland & Cambridge, 32 pp.

    Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33(7): 1870–1874.
    DOI: 10.1093/molbev/msw054View Article Google Scholar

    Miller, M.A., W. Pfeiffer and T. Schwartz. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE). IEEE, Piscataway, pp. 45–52.
    DOI: 10.1109/GCE.2010.5676129View Article Google Scholar

    Mols, J.B. and P.J.A. Ke?ler. 2000. Revision of the genus Phaeanthus (Annonaceae). Blumea 45(1): 205–233.

    Mols, J.B. and P.J.A. Ke?ler. 2003. The genus Miliusa (Annonaceae) in the Austro-Malesian area. Blumea 48(3): 421–462.
    DOI: 10.3767/000651903X489384View Article Google Scholar

    Mols, J.B., D.L.V. Co, B. Gravendeel, L.W. Chatrou, M.D. Pirie, R.W.J.M. van der Ham, E.J. van Marle, and P.J.A. Ke?ler. 2004. Morphological character evolution in the miliusoid clade (Annonaceae). In: Mols, J.B. (ed.) From Miliusa to Miliuseae to Miliusoid: identifying clades in Asian Annonaceae. PhD Thesis, Leiden University, The Netherlands, pp. 37–75.

    Nguyen, L.T., H.A. Schmidt, A. von Haeseler and B.Q. Minh. 2015. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol. Biol. Evol. 32(1): 268–274.
    DOI: 10.1093/molbev/msu300View Article Google Scholar

    Oliver, D. 1887. Plate 1561. Phaeanthus lucidus Oliv. Anonaceae. Tribe Mitrephoreae. In: Hooker, J.D. (ed.) Hooker’s icones plantarum, vol. 16 (part 3). Williams & Norgate, London & Edinburgh, pl. 1551–1575.

    Pirie, M.D., L.W. Chatrou, J.B. Mols, R.H.J. Erkens and J. Oosterhof. 2006. ‘Andean-centred’ genera in the short-branch clade of Annonaceae: testing biogeographical hypotheses using phylogeny reconstruction and molecular dating. J. Biogeogr. 33(1): 31–46.
    DOI: 10.1111/j.1365-2699.2005.01388.xView Article Google Scholar

    Posada, D. and K.A. Crandall. 1998. Modeltest: testing the model of DNA substitution. Bioinformatics 14(9): 817–818.
    DOI: 10.1093/bioinformatics/14.9.817View Article Google Scholar

    Photikwan, E., A. Damthongdee, H. Jongsook and T. Chaowasku. 2021. Artabotrys angustipetalus (Annonaceae), a new species from Thailand, including a plastid phylogeny and character evolutionary analyses of thorn occurrence in Artabotrys. Willdenowia 51(1): 69–82.
    DOI: 10.3372/wi.51.51106View Article Google Scholar

    Rambaut, A., M. Suchard and A. Drummond. 2013. Tracer, version 1.6. Available from: http://tree.bio.ed.ac.uk/software/tracer (accessed 18 May 2017).

    Ronquist, F., M. Teslenko, P. van der Mark, D.L. Ayres, A. Darling, S. H?hna, B. Larget, L. Liu, M.A. Suchard and J.P. Huelsenbeck. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61(3): 539–542.
    DOI: 10.1093/sysbio/sys029View Article Google Scholar

    Simmons, M.P. 2004. Independence of alignment and tree search. Mol. Phylogenet. Evol. 31(3): 874–879.
    DOI: 10.1016/j.ympev.2003.10.008View Article Google Scholar

    Simmons, M.P. and H. Ochoterena. 2000. Gaps as characters in sequence-based phylogenetic analyses. Syst. Biol. 49(2): 369–381.
    DOI: 10.1080/10635159950173889View Article Google Scholar

    Sinclair, J. 1955. A revision of the Malayan Annonaceae. Gard. Bull. Singapore 14(2): 149–516.

    Staden, R., K.F. Beal and J.K. Bonfield. 2000. The Staden package, 1998. In: Misener, S. and S.A. Krawetz (eds.) Bioinformatics methods and protocols. Methods Mol. Biol. Humana Press, Totowa, pp. 115–130.
    DOI: 10.1385/1-59259-192-2:115View Article Google Scholar

    Tavar?, S. 1986. Some probabilistic and statistical problems in the analysis of DNA sequences. Lectures Math. Life Sci. 17: 57–86.

    Turner, I.M. 2018. Annonaceae of the Asia-Pacific region: names, types and distributions. Gard. Bull. Singapore 70(2): 409–744.
    DOI: 10.26492/gbs70(2).2018-11View Article Google Scholar

    Turner, I.M. and J.F. Veldkamp. 2011. William Roxburgh’s eye plant and its relevance in the nomenclature of Phaeanthus (Annonaceae). Kew Bull. 66(4): 571–579.
    DOI: 10.1007/s12225-011-9307-7View Article Google Scholar

    Wiens, J.J. 1998. Combining data sets with different phylogenetic histories. Syst. Biol. 47(4): 568–581.
    DOI: 10.1080/106351598260581View Article Google Scholar

    Xue, B., Y.H. Tan and X.E. Ye. 2016. The identity of Polyalthia florulenta (Annonaceae): a second species of Wangia in China. Phytotaxa 283(2): 163–171.
    DOI: 10.11646/phytotaxa.283.2.5View Article Google Scholar

    Yang, Z. and B. Rannala. 1997. Bayesian phylogenetic inference using DNA sequences: a Markov Chain Monte Carlo method. Mol. Biol. Evol. 14(7): 717–724.
    DOI: 10.1093/oxfordjournals.molbev.a025811View Article Google Scholar