Research Paper

Seed biology and early seedling developmental traits of Clidemia hirta, an invasive species of Sri Lankan rainforests compared to two native species sharing the same habitat

B.R. Chandima P. Samarasinghe, K.M.G. Gehan Jayasuriya, A.M. Thilanka A. Gunaratne, Kingsley W. Dixon, Mahesh Senanayaka

Published on: 11 January 2022

Page: 73 - 82

DOI: 10.6165/tai.2022.67.73

Abstract

Comparative studies on invasive species with native species sharing the same habitats are valuable in controlling invasive plants. This study compared seed biology and seedling development traits of two native species, Melastoma malabathricum L and Plectranthus kanneliyensis Willemse, and the invasive species Clidemia hirta (L.) D. Don, which share a tropical rainforest habitat in Sri Lanka. In particular, the adaptability of these species to their habitat was assessed. Seed moisture content, dormancy status and desiccation sensitivity were also tested. Seedling growth and survival was observed through greenhouse experiments. Seeds of all the study species germinated >50% in light/dark conditions, but none germinated in complete darkness or under green light. Time taken to reach 50% germination (T50) and final germination percentage suggested that C. hirta and P. kanneliyensis seeds are non-dormant while M. malabathricum seeds are dormant. All study species produced desiccation tolerant seeds. Under all light conditions, seeds of C. hirta recorded the highest seed germination and seedling survival percentage, while P. kanneliyensis had the highest growth rate. Interspecies difference in photosynthesis efficiency was not observed. These experiments demonstrated that the study species have different strategies to adapt to their shared habitat. Furthermore, higher germination percentage and higher survival may be the key factors that determines the invading ability of C. hirta, enabling them to outcompete M. malabathricum and P. kanneliyensis. Hence, immediate actions must be taken to prevent further invasion of C. hirta into disturbed sites in tropical rainforest regions.

Keyword: Invasive species, native species, seed dormancy, seed germination, seedling survival

Literature Cited

Bambaradeniya, C.N.B. 2002. The status and impacts of alien invasive species in Sri Lanka. Zoos Print J. 17(11): 930?935.
DOI: 10.11609/JoTT.ZPJ.17.11.930-5View Article Google Scholar

Baskin, C.C. and J.M. Baskin. 2014. Seeds: ecology, biogeography and evolution of dormancy and germination. 2nd ed. Academic Press, San Diego, USA. 1600 pp.

Bazzas, F.A. and S.T.A. Pickett. 1980. Physiological ecology of Tropical Succession: A comparative review. Annu. Rev. Ecol. Syst. 11(1): 287?310.
DOI: 10.1146/annurev.es.11.110180.001443View Article Google Scholar

Bradshaw, C.J., N.S. Sodhi and B.W. Brook. 2009. Tropical turmoil: a biodiversity tragedy in progress. Front. Ecol. Environ. 7(2): 79?87.
DOI: 10.1890/070193View Article Google Scholar

Cameron, T.C., H.J. Wearing, P. Rohani and S.M. Sait. 2007. Two-species asymmetric competition: effects of age structure on intra-and interspecific interactions. J. Anim. Ecol. 76(1): 83?93.
DOI: 10.1111/j.1365-2656.2006.01185.xView Article Google Scholar

Cano L., J. Escarr?, I. Fleck, J.M. Blanco-Moreno and F.X. Sans. 2008. Increased fitness and plasticity of an invasive species in its introduced range: a study using Senecio pterophorus. J. Ecol. 96(3): 468?476.
DOI: 10.1111/j.1365-2745.2008.01363.xView Article Google Scholar

Castro J., R. Zamora, J.A. Hodar and J.M. G?mez. 2005. Ecology of seed germination of Pinus sylvestris L. at its southern, Mediterranean distribution range. For. Syst. 14(2): 143?152.
DOI: 10.5424/srf/2005142-00879View Article Google Scholar

Cervera J.C. and V. Parra-Tabla. 2009. Seed germination and seedling survival traits of invasive and non-invasive congeneric Ruellia species (Acanthaceae) in Yucatan, Mexico. Plant Ecol. 205(2): 285?293.
DOI: 10.1007/s11258-009-9617-0View Article Google Scholar

Chapple D.G., S.M. Simmonds and B.B. Wong. 2012. Can behavioral and personality traits influence the success of unintentional species introductions? Trends Ecol. Evol. 27(1): 57?64.
DOI: 10.1016/j.tree.2011.09.010View Article Google Scholar

Cross, A.T., M.D. Barrett, S.R. Turner, K.W. Dixon and D.J. Merritt. 2019. Seed-dormancy depth is partitioned more strongly among habitats than among species in tropical ephemerals. Aust. J. Bot. 66(3): 230?242.
DOI: 10.1071/BT17244View Article Google Scholar

D’Antonio, C.M. 2000. Fire, plant invasions, and global changes. In: Mooney H. A. and Hobbs R. J. (eds.). Invasive species in a changing world. Island Press, Washington, DC, USA. 65?93pp

DeWalt, S.J., J.S. Denslow and J.L. Hamrick. 2004a. Biomass allocation, growth and photosynthesis of genotypes from native and introduced ranges of the tropical shrub Clidermia hirta. Oecologia 138(4): 521?531.
DOI: 10.1007/s00442-003-1462-6View Article Google Scholar

DeWalt, S.J., J.S. Denslow and K. Ickes. 2004b. Natural-enemy release facilitates habitat expansion of the invasive tropical shrub, Clidermia hirta. Ecology 85(2): 471?483.
DOI: 10.1890/02-0728View Article Google Scholar

Divisek, J., M. Chytry, B. Beckage, N.J. Gotelli, Z. Lososova, P. Pysek, D.M. Richardson and J. Molofsky. 2018. Similarity of introduced plant species to native ones facilitates naturalization, but differences enhance invasion success. Nat. Commun. 9(1): 4631.
DOI: 10.1038/s41467-018-06995-4View Article Google Scholar

Donohue, K. 2003. Setting the stage: phenotypic plasticity as habitat selection. Int. J. Plant Sci. 164(S3): S79?S92.
DOI: 10.1086/368397View Article Google Scholar

Donohue, K. 2005. Niche construction through phenological plasticity: life history dynamics and ecological consequences. New Phytol. 166(1): 83?92.
DOI: 10.1111/j.1469-8137.2005.01357.xView Article Google Scholar

Donohue, K., R.R. de Casas, L. Burghardt, K. Kovach and C.G. Willis. 2010. Germination, post germination adaptation, and species ecological ranges. Annu. Rev. Ecol. Evol. Syst. 41(1): 293?319.
DOI: 10.1146/annurev-ecolsys-102209-144715View Article Google Scholar

Faravani, M. and B.B. Bakar. 2007. Effects of light on seed germination, growth pattern of straits Rhododendron (Melastoma malabathricum L.). J. Agric. Biol. Sci. 2(3): 1?5.

Fenner, M.K., M. Fenner and K. Thompson. 2005. The ecology of seeds. Cambridge University Press.
DOI: 10.1017/CBO9780511614101View Article

Finegan, B. 1984. Forest succession. Nature 312(5990): 109?114.
DOI: 10.1038/312109a0View Article Google Scholar

Forbis, T.A. 2010. Germination phenology of some Great Basin native annual forb species. Plant Species Biol. 25(3): 221?230.
DOI: 10.1111/j.1442-1984.2010.00289.xView Article Google Scholar

Gioria M., P. Py?ek and B.A. Osborne. 2018. Timing is everything: does early and late germination favor invasions by herbaceous alien plants? J. Plant Ecol. 11(1): 4?16.
DOI: 10.1093/jpe/rtw105View Article Google Scholar

Global Invasive Species Database 2020. Downloaded from http://www.iucngisd.org/gisd/100_worst.php on 22-12-2020.

Guido, A., D. Hoss and V.D. Pillar. 2017. Exploring seed to seed effects for understanding invasive species success. Perspect. Ecol. Conserv. 15(3), 234?238.
DOI: 10.1016/j.pecon.2017.07.006View Article Google Scholar

Hejda, M., P. Py?ek and V. Jaro??k. 2009. Impact of invasive plants on the species richness, diversity and composition of invaded communities. J. Ecol. 97(3): 393?403.
DOI: 10.1111/j.1365-2745.2009.01480.xView Article Google Scholar

International Seed Testing Association (ISTA). 2018. International Rules for Seed Testing, Bassersdorf, Switzerland, pp 278.

Jordan, N.R., D.L. Larson and S.C. Huerd. 2008. Soil modification by invasive plants: effects on native and invasive species of mixed-grass prairies. Biol. Invasions 10(2): 177?190.
DOI: 10.1007/s10530-007-9121-1View Article Google Scholar

Kariyawasam, C.S., L. Kumar and S.S. Ratnayake. 2020. Potential Risks of Plant Invasions in Protected Areas of Sri Lanka under Climate Change with Special Reference to Threatened Vertebrates. Climate 8(4): 51?71.
DOI: 10.3390/cli8040051View Article Google Scholar

Krupnick, G.A. 2013. Conservation of tropical plant biodiversity: what have we done, where are we going? Biotropica 45(6): 693-708.
DOI: 10.1111/btp.12064View Article Google Scholar

Lal, N. and P. Sachan. 2017. Effect of different visible light wavelengths on seed germination and photosynthetic pigment contents in Vigna unguiculata (L.)Walp. Indian J. Biol. 4(2): 132?136.

Le, C., K. Fukumori, T. Hosaka, S. Numata, M. Hashim and T. Kosaki. 2018. The distribution of an invasive species, Clidemia hirta along roads and trails in Endau Rompin National Park, Malaysia. Trop. Conserv. Sci. 11: 1?9.
DOI: 10.1177/1940082917752818View Article Google Scholar

Li, L., L. Ren, Y. Bai, D. Lecain, D. Blumenthai and J. Morgan. 2018. Seed traits and germination of native grasses and invasive forbs are largely insensitive to parental temperature and Co3 concentration. Seed Sci. Res. 28(4): 303?311.
DOI: 10.1017/S0960258518000314View Article Google Scholar

Marambe, B., C. Bambaradeniya, D.K. Pushpa Kumara and N. Pallewatta. 2001. Human dimensions of invasive alien species in Sri Lanka. In J.A. McNeely (ed.), The great reshuffling, human dimensions of invasive aliean species. pp 135-145. IUCN Publications Services Unit, Cambridge, UK.

Mal?kov?, L., O. Mudr?k and J. Klime?ov?. 2012. Adventitious sprouting enables the invasive annual herb Euphorbia geniculata to regenerate after severe injury. Ecol. Res. 27(5): 841?847.
DOI: 10.1007/s11284-012-0960-6View Article Google Scholar

Martinez-Ghersa, M.A., C.M. Ghersa, R.L. Bench-Arnold, R.M. Donough and R.A. Sanchez. 2000. Adaptive traits regulating dormancy and germination of invasive species. Plant Species Biol. 15(2): 127?137.
DOI: 10.1046/j.1442-1984.2000.00033.xView Article Google Scholar

Maxwell, K. and G.N. Johnson. 2000. Chlorophyll fluorescence - a practical guide. J. Exp. Bot. 51(345): 659?668.
DOI: 10.1093/jexbot/51.345.659View Article Google Scholar

Medeiros, A.C. 2004. Phenology, reproductive potential, seed dispersal and predation, and seedling establishment of three invasive plant species in a Hawaiian rain forest. Doctoral dissertation, Louisiana State University, USA.

Meyer, W.M. and R.H. Cowie. 2010. Invasive Temperate Species are a Threat to Tropical Island Biodiversity. Biotropica 42(6): 732?738.
DOI: 10.1111/j.1744-7429.2010.00629.xView Article Google Scholar

Moran, E.V., A. Reid and J.M. Levine. 2017. Population genetics and adaptation to climate along elevation gradients in invasive Solidago canadensis. PLos One 12(9): e0185539.
DOI: 10.1371/journal.pone.0185539View Article Google Scholar

Moravcov?, L., P. Py?ek, V. Jaro??k and J. Pergl. 2015. Getting the right traits: reproductive and dispersal characteristics predict the invasiveness of herbaceous plant species. PloSone. 10(4): e0123634.
DOI: 10.1371/journal.pone.0123634View Article Google Scholar

Mullah, C.J.A., K. Klanderud, ?. Totland and D. Odee. 2014. Community invasibility and invasion by non-native Fraxinus pennsylvanica trees in a degraded tropical forest. Biol. Invasions 16(12): 2747?2755.
DOI: 10.1007/s10530-014-0701-6View Article Google Scholar

Phartyal, S.S., S. Rosbakh, C. Ritz and P. Poschlod. 2019. Ready for change: seed traits to the high adaptability of mudflat species to their unpredictable habitat. J. Veg. Sci. 31(2): 331?342.
DOI: 10.1111/jvs.12841View Article Google Scholar

Peters, H.A. 2001. Clidemia hirta invasion at the pasoh forest reserve: an unexpected plant invasion in an undisturbed tropical forest 1. Biotropica 33(1): 60?68.
DOI: 10.1111/j.1744-7429.2001.tb00157.xView Article Google Scholar

Pritchard, H.W., C.B. Wood, S. Hodges and H.J. Vautier. 2004. 100-seed test for desiccation tolerance and germination: a case study on eight tropical palm species. Seed Sci. Technol. 32(2): 393?403.
DOI: 10.15258/sst.2004.32.2.11View Article Google Scholar

Simmonds, H.W. 2009. The biological control of the weed Clidermia hirta D. Don., in Fiji. Bull. Entomol. Res. 24(3): 345?348.
DOI: 10.1017/S0007485300031655View Article Google Scholar

Singhakumara, B.P., R.S.P. Uduporuwa and P.M.S. Ashton. 2000. Soil Seed Banks in Relation to Light and Topographic Position of a Hill Dipterocarp Forest in Sri Lanka1. Biotropica 32(1): 190?196.
DOI: 10.1111/j.1744-7429.2000.tb00461.xView Article Google Scholar

Smith, C.W. 1992. Distribution, status, phenology, rate of spread, and management of Clidemia in Hawaii. Alien plant invasions in native ecosystems of Hawaii: management and research. University of Hawaii Cooperative National Park Resources Studies Unit, Honolulu. 241?253.

Subramanyam, K. 1948. An embryological study of Melastoma malabathricum L. J. Indian Bot. Soc. 27: 11?19.

Tiebel, K., F. Huth and S. Wagner. 2018. Soil seed banks of pioneer tree species in European temperate forests: a review. iForest 11(1): 48?57.
DOI: 10.3832/ifor2400-011View Article Google Scholar

Tweddle, J.C., J.B. Dickie, C.C. Baskin and J.M. Baskin. 2003. Ecological aspects of seed desiccation sensitivity. J. Ecol. 91(2): 294?304.
DOI: 10.1046/j.1365-2745.2003.00760.xView Article Google Scholar

Waddell, E.H., D.S. Chapman, J.K. Hill, M. Hughes, A. Bin Sailim, J. Tangah, and L.F. Banin. 2020. Trait filtering during exotic plant invasion of tropical rainforest remnants along a disturbance gradient. Funct. Ecol. 34(12): 2584?2597.
DOI: 10.1111/1365-2435.13679View Article Google Scholar

Wang, Y., L. Lai, L. Jiang, P. Zhuang, Y. Zhang, Baskin, J.M. and C.C. Baskin. 2014. Geographic variation in seed traits within and among forty-two species of Rhododendron (Ericaceae) on the Tibetan plateau: relationship with altitude, habitat, plant height and phylogeny. Ecol. Evol. 4(10): 1913?1923.
DOI: 10.1002/ece3.1067View Article Google Scholar

Wijesundara, S. 2010. Defining invasive alien species. Invasive alien species in Sri Lanka - strengthening capacity to control their introduction and spread, 1?6.

Wyse, S.V. and J.B. Dickie. 2018. Taxonomic affinity, habitat and seed mass strongly predict seed desiccation response: a boosted regression trees analysis based on 17539 species. Ann. Bot. 121(1): 71?83.
DOI: 10.1093/aob/mcx128View Article Google Scholar

Wester, L.L. and H.B. Wood. 1977. Koster's curse (Clidemia hirta), a weed pest in Hawaiian forests. Environ. Conserv. 4(1): 35?41.
DOI: 10.1017/S0376892900024991View Article Google Scholar

Wolny, E., A. Betekhtin, M. Rojek, A. Braszewska-Zalewska, J. Lusinska and R. Hasterok. 2018. Germination and the early stages of seedling development in Brachypodium distachyon. Int. J. Mol. Sci. 19(10): 2916.
DOI: 10.3390/ijms19102916View Article Google Scholar

Xu, X., L. Wolfe, J. Diez, Y. Zheng, H. Guo and S. Hu. 2019. Differential germination strategies of native and introduced populations of the invasive species Plantago virginica. NeoBiota 43: 101?118.
DOI: 10.3897/neobiota.43.30392View Article Google Scholar

Yang, S.Z. 2001. A new record and invasive species in Taiwan, Clidermia hirta (L.) D. Don. Taiwania 46(3): 232-237.
DOI: 10.6165/tai.2001.46(3).232View Article Google Scholar

Yu, S., M. Sternberg, P. Kutiel and H. Chen. 2007. Seed mass, shape, and persistence in the soil seed bank of Israeli coastal sand dune flora. Evol. Ecol. Res. 9(2): 325?340.

Zhang, J., E. Siemann, B. Tian, W. Huang, and J. Ding. 2020. Differences in seed properties and germination between native and introduced populations of Triadica sebifera. J. Plant Ecol. 13(1): 70?77.
DOI: 10.1093/jpe/rtz048View Article Google Scholar

Zerbe, S. 2001. On the ecology of Sorbus aucuparia (Rosaceae) with special regard to germination, establishment and growth. Pol. Bot. J. 46(2): 229?239.