@Article{taiwania197924170,
AUTHOR = {Wen-Hui Chen, William Cockburn, Herbert Edward Street},
TITLE = {Preliminary experiments on freeze preservation of sugarcane cells},
JOURNAL = {Taiwania},
VOLUME = {24},
YEAR = {1979},
ISSUE = {1},
PAGES = {70-74},
URL = {https://taiwania.ntu.edu.tw/abstract/615},
ABSTRACT = {A preliminary investigation on freeze preservation of sugarcane cell cultures indicated that sugarcane cultures frozen at a rate of 1C/min to -100C after treating with 10% (v/v) glycerol +5% (v/v) dimethylsulfoxide (DMSO), and thawed in a water bath at 37 C, yielded up to 30% viable cells. 10% glycerol was found to be more effectived cryoprotectant for sugarcane cells than 5% DMSO.  Rapid thawing in a water bath at 37C gave the highest viability of sugarcane cells recovered following freezing.  Frozen sugarcane cells stored in the liquid nitrogen refrigerator, up to 4 months, indicate that no decrease in cell viability was observed after thawing as compared with control.},
DOI = {10.6165/tai.1979.24.70}
}