@Article{taiwania1990352112, AUTHOR = {San-San Tsay, His-Hua Wang}, TITLE = {Isolation and Characterization of Bdellovibrio Parasitic to Gram-Negative Fish Pathogenic Bacterium Aeromonas hydrophila}, JOURNAL = {Taiwania}, VOLUME = {35}, YEAR = {1990}, ISSUE = {2}, PAGES = {112-126}, URL = {https://taiwania.ntu.edu.tw/abstract/72}, ABSTRACT = {One stain of bacteria parasite-Bdellovibrio was isolated from cultured fish ponds in Taiwan. This stain is able to parasitize on Aeromonas hydrophila, designated as Bdellovibrio A-1. The morphology of host and parasite were examined by means of electron microscopy. The effect of medium composition, initial pH of medium, incubation temperature, addition of divalent cation and kinds and concentration of host bacteria on the growth of Bdellovibrio were investigated. The optimum temperature for the growth of Bdellovibrio in liquid medium was 25-29 C. the optimum initial pH of medium was pH 6.5. growth of the parasite was dependent. Upon the addition of Ca2+ and Mg2+. The highest lytic action of Bdellovibrio was obtained by using a higher concentration of fish pathogenic bacteria (4 10 cells/ml) as host.}, DOI = {10.6165/tai.1990.35.112} }