@Article{taiwania199237167,
AUTHOR = {W.S. Chen, S.C. Tso, Y.F. Huang and Y.R. Chen},
TITLE = {Acid phosphatase in rice seedlings},
JOURNAL = {Taiwania},
VOLUME = {37},
YEAR = {1992},
ISSUE = {1},
PAGES = {67-77},
URL = {https://taiwania.ntu.edu.tw/abstract/212},
ABSTRACT = {Acid phosphatase (EC 3. 1. 3. 2.) extracted from rice seedlings consisted of ten kinds of isozymes based on separation by polyacrylamide gel electrophoresis. One isozyme was purified through ammonium sulfate precipitation, Ultrogel gel filtration, DEAE-trisacryl chromatography and chromatofocusing. This enzyme had a high affinity to p-nitrophenyl phosphate. The molecular weight of this isozyme was around 130kD examined with FPLC and it consisted of heterodimer with molecular weight of 70kD and 62kD. The purified isozyme had a pH optimum at 5.0 and its Km (p-nitrophenyl phosphate) was 0.69 mM. Mg++ and Mn++ enhanced the activity, but heave metal ions were inhibitory. NaF and Na2MoO4 also had an inhibitory effect on the isozyme.},
DOI = {10.6165/tai.1992.37.67}
}