@Article{taiwania1996413244,
AUTHOR = {Yih-Yuan Chang  and Yung-Reui Chen},
TITLE = {Immunofluorescent Localization of 24 kDa Protein in Luffa cylindrica Phloem Exudate},
JOURNAL = {Taiwania},
VOLUME = {41},
YEAR = {1996},
ISSUE = {3},
PAGES = {244-251},
URL = {https://taiwania.ntu.edu.tw/abstract/352},
ABSTRACT = {A 24 kDa phloem protein was isolated from phloem exudate of Luffa cylindrica fruits by the method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Polyclonal antibodies obtained from rabbit serum recognized both the native protein and denatured protein subunits. Immunofluorescent study was done by a FITC-conjugated goat-anti-rabbit IgG. Immunolabelled sections of pepo show that the antibodies strongly bound with 24 kDa protein in the bicollateral phloem tissues of vascular bundle. The intensity of green fluorescence is related to the degree of phloem differentiation. Similar results were also observed in stems and petiols of leaves.},
DOI = {10.6165/tai.1996.41.244}
}