@Article{taiwania1997423180, AUTHOR = {Ming-Chuan Wang}, TITLE = {Separation of a Phloem Specific 42.5kDa Protein with Two-Dimensional Gel}, JOURNAL = {Taiwania}, VOLUME = {42}, YEAR = {1997}, ISSUE = {3}, PAGES = {180-188}, URL = {https://taiwania.ntu.edu.tw/abstract/365}, ABSTRACT = {Phloem exudates leaking from Luffa cylindrica fruits by a simple razor blade cutting were mixed with the extraction medium containing antioxidant and rapidly separated by 2-dimentional gel, i. E., native and sodium dodecylsulfate(SDS) polyacrylamide gel electrophoresis(PAGE). A major protein of 85kDa occupying 28.2% of total protein had 5 bands on denatured gel. One of them with molecular weight at 42.5kDa was complementally identified by reversed-phase high performance liauid chromatography and silver stained-band on SDS-gel.}, DOI = {10.6165/tai.1997.42.180} }