@Article{taiwania1998434295,
AUTHOR = {Shih-Tong Jeng and Shiau-Wei Lee},
TITLE = {Effects of the Concentration of Nucleoside Triphosphates on the Transcription of Bacteriophage T7 RNA Polymerase in the Linearized and Supercoiled DNA Templates},
JOURNAL = {Taiwania},
VOLUME = {43},
YEAR = {1998},
ISSUE = {4},
PAGES = {295-306},
URL = {https://taiwania.ntu.edu.tw/abstract/177},
ABSTRACT = {The thr attenuator is a Dna element containing a G+C-rich dyad symmetry followed by a run of poly(dT), and is reported to be a rho-independent terminator.The effects of the concentrations of nucleoside triphosphates on the transcription of bacteriophage T7 polymerases were analyzed at the thr attenuator in the supercoiled and linearized DNA templates. T7 RNA polymerase showed high termination efficiency in the low concentration of nucleosied triphosphates at the thr attenuator in the both linearized and supercoiled templates. The thr attenuator in the supercoiled DNA template always caused more T7 RNA polymerase to cease transcription than that in the linearized DNA template. Also, when the concentration of ATP is limited from 5 μM to 150 μM, the transcrip production by t& RNA polymerase was at least two folds more than that in the limited supply of any other nucleoside triphosphates. This indicates that ATP may compete with GTP in affecting transcription of T7 RNA polymerase.},
DOI = {10.6165/tai.1998.43(4).295}
}