@Article{taiwania200247124,
AUTHOR = {Yu-Chi Chen, Hung-Shu Chang, Winston T. K. Cheng, and Shih-Tong Jeng},
TITLE = {Cloning and Expression of the Ipomoelin Gene from Sweet Potato},
JOURNAL = {Taiwania},
VOLUME = {47},
YEAR = {2002},
ISSUE = {1},
PAGES = {24-30},
URL = {https://taiwania.ntu.edu.tw/abstract/308},
ABSTRACT = {A full-length cDNA encoding ipomoelin protein was isolated from an enriched cDNA library, which contained wound-inducible cDNAs subtracted from that of unwounded sweet potato. Ipomoelin gene can be induced by methyl jasmonic acid, but independent of ABA. In prior studies, the expression of ipomoelin gene was found to be inhibited by salicylic acid, and related to protein kination and phosphorylation. In this study, the deduced protein sequence of ipomoelin was compared with amino acid sequences of other proteins, and it exhibited a high homology with lectin. In order to study further the function of ipomoelin, the ipomoelin gene was constructed and transformed into a bacteria expression system. The result shows that the ipomoelin protein could be over-expressed in E.coli one hour after IPTG induction and reached a maximum four hours after induction.},
DOI = {10.6165/tai.2002.47(1).24}
}