@Article{taiwania2004493159,
AUTHOR = {Hsiu-Hui Shih},
TITLE = {Neutralization of White Spot Syndrome Virus by Monoclonal Antibodies against Viral Envelope Proteins},
JOURNAL = {Taiwania},
VOLUME = {49},
YEAR = {2004},
ISSUE = {3},
PAGES = {159-165},
URL = {https://taiwania.ntu.edu.tw/abstract/419},
ABSTRACT = {Two monoclonal antibodies (MAbs) recognizing envelope proteins of the white spot
syndrome virus (WSSV), 6E1 against VP28 and 3E8 against VP19, were applied to demonstrate their neutralizing ability to this virus by using both in vitro and in vivo assays. Mixtures of MAb 6E1 with virus filtrate were inoculated into the primary explant monolayer culture derived from the lymphoid Oka organs of Penaeus monodon. Mab was likely to neutralize the infectivity of virus to monolayer since cytopathic effects were apparently blocked in experiment group. WSSV was titrated using Blue-Cell ELISA and the neutralizing index was calculated to be 6.90 for 6EI and 5.83 for 3E8.  Neutralized virus fluids injected intramuscularly into post larvae of P. monodon. The shrimp in the positive control, which were injected with WSSV only showed an increasing mortality and a 100% mortality was reached at day 34, whereas no shrimp died in the negative control. The mortality for 6E1 was 6.7% and for 3E8 was 13.3%. These results suggest that Mabs recognizing the WSSV envelope proteins could neutralize viral infectivity to both cultured cells and shrimp.},
DOI = {10.6165/tai.2004.49(3).159}
}