@Article{taiwania20115611, AUTHOR = {Yoshimasa Yamamoto}, TITLE = {Elucidating the Adhesion and Growth of the Green Alga Scenedesmus acutus under Culture Conditions by Using Ultrasonic Treatment}, JOURNAL = {Taiwania}, VOLUME = {56}, YEAR = {2011}, ISSUE = {1}, PAGES = {1-6}, URL = {https://taiwania.ntu.edu.tw/abstract/1050}, ABSTRACT = {This study examined the feasibility of using ultrasonic treatment to investigate the adhesion and growth of the green alga Scenedesmus acutus. The number of S. acutus cells in a colony ranged from one to eight, with a median of four. The percentages of 1- and 2-cell colonies increased with the duration of sonication. Although unaffected by a short-term (30 s) sonication, the ratio of colony formation of S. acutus on agar plates was significantly decreased by extended (60 s) sonication. Additionally, short-term sonication was effective in detaching adhered algal cells from the test tube wall without adversely affecting their growth, implying its potential usefulness in monitoring the growth of S. acutus. S. acutus cells adhered to the test tube wall within several hours, with the ratio of adhered cells reaching the maximum level (ca. 90 %) within 24 h. At 25°C, the cells usually adhered to the test tube wall rapidly including the conditions at which the growth of S. acutus was suppressed, such as a low nutrient concentration and weak irradiance. However, adhesion proceeded slowly at 15°C, implying that temperature is essential to regulating the adhesion of S. acutus.}, DOI = {10.6165/tai.2011.56(1).1} }