@Article{taiwania201257127,
AUTHOR = {Vishal Awasthi, Virendra Nath, Neerja Pande and Ashish Kumar Asthana},
TITLE = {Morphogenetic Studies and In vitro Propagation of Two Mosses: Philonotis thwaitesii Mitt. and Brachythecium plumosum (Hedw.) B.S.G.},
JOURNAL = {Taiwania},
VOLUME = {57},
YEAR = {2012},
ISSUE = {1},
PAGES = {27-36},
URL = {https://taiwania.ntu.edu.tw/abstract/1113},
ABSTRACT = {Well differentiated gametophytes of Philonotis thwaitesii Mitt., an acrocarpous moss and Brachythecium plumosum (Hedw.) B.S.G., a pleurocarpous moss have been raised in vitro by inoculating their spores into a range of concentrations of inorganic media. Half-strength Knop’s macronutrients + Nitsch’s trace elements with 10 ppm ferric citrate was found the most suitable for growth and multiplication of these species in continuous light of 4,000-5,500 lux and at 21 ± 2ºC temperature. Protonemal buds developed on caulonema in P. thwaitesii while on chloronema in B. plumosum. Sucrose stimulated caulonemal differentiation, abundant rhizoid production and a few but much robust gametophyte development. Emergence of hyaline rhizoids like filaments has been observed in P. thwaitesii upon contact of gametophores with solid substratum while true rhizoids all along the length of gametophytes of B. plumosum emerges upon placing them horizontally on culture medium. Variable growth forms (weft-like branched as well as erect unbranched habit) have been observed in both species in culture conditions. Sub-culturing of P. thwaitesii gave rise to new population via passing through chloronemal and caulonemal stage while B. plumosum directly regenerated into new gametophytes by-passing protonemal stage. In vitro raised plants were acclimatized and transferred to soil in order to their further proliferation.},
DOI = {10.6165/tai.2012.57(1).27}
}