%0 Journal Article
%A Yung-Reui Chen
%T Complementary Studies of Tobacco Protoplast with Freeze-Fracture and Thin Section Techniques
%D 1986
%J Taiwania
%V 31
%N 1
%P 129-141
%U https://taiwania.ntu.edu.tw/abstract/554
%X     Tobacco protoplast enzymatically isolated from suspension culture were studied. Isolated protoplast showed intact plasma membrane surrounded the outer surface, but sometimes membrane with breb was also observed. Endoplasmic reticulum was abundant and 3 to 10 layers of cisternae in parallel arrangement ofren occurred. Invaginations of cytoplasmic membrane with sime organelles in it were also found. Oil drops prominently occurred in isolated protoplasts with different characters in staining properties and structure. The central and large vacuoles disappeared and small vacuoles tremendously increased in number. Nucleus located in the center of protoplasts with a distinct nucleolus. Annuli on the surface of nuclear envelope were numerous (35/um^2). Plastids were different in shape and size. Structural patterns of plastids are more diverse than that of mitochondria. Stereopairs showed the three dimensional structures of protoplasts were all identical to that observed in thin section.
%M doi:10.6165/tai.1986.31.129