%0 Journal Article
%A Suu-Mei Pan
%A Yuan-Yeu Yau
%T Characterization of superoxide dismutase in Arabidopsis
%D 1992
%J Taiwania
%V 37
%N 1
%P 58-66
%U https://taiwania.ntu.edu.tw/abstract/211
%X Superoxide dismutase (SOD: EC 1.15.1.1) from different plant parts of Arabidopsis and paraquat-treated leaves were characterized using polyacrylamide gel electrophoresis. Five different forms of SOD were identified by their sensitivity to cyanide and hydrogen peroxide. The molecular weight of Mn-SOD, Fe-SOD and CuZn-SODs was estimated to be 80KD, 50KD, 35KD, 25KD, 20KD and 15KD, respectively. A similar pattern of Mn-, Fe- and CuZn-SOD was observed in all plant parts. The pIs of the SODs were determined to be 3.8, 4.5, 4.8 and 5.35 by isoelectric focusing. CuZn-SODs contributed the major activity of total SOD. Only the CuZn-SOdIII activity showed an apparent increase following the Arabidopsis plants treated with paraquat.
%M doi:10.6165/tai.1992.37.58