%0 Journal Article
%A Shu-Mei Pan
%A Guan-Bor Hwang
%A Daw-Shyng Wei and Kai-Wun Yeh
%T ISOLATION AND CHARACTERIZATION OF A cDNA FOR CuZn-SUPEROXIDE DISMUTASE OF RICE
%D 1995
%J Taiwania
%V 40
%N 1
%P 1-12
%U https://taiwania.ntu.edu.tw/abstract/868
%X RT-PCR was used for the SOD-DNA amplification from rice total RNA in a two-step reaction. Using this PCR-made SOD-DNA as the probe to screen a rice λgt11 cDNA library, four putative clones of CuZnSOD were isolated. The clone having the largest insert was subcloned and sequenced. This cDNA clone had a full-length open reading frame of 459bp corresponding to 152 amino acid residues and the derived amino acid sequence from this clone matched the cytosolic CuZnSOD isozyme. Its amino acid sequence showed approximately 99 and 86% identity to rice and corn CuZnSOD sequences. Northern blot hybridization showed that the heat-shock, drought-treated rice seedlings had the enhanced transcripts of CuZnSOD. Genomic Southern analysis indicated that CuZnSODs in rice was encoded by multigenes.
%M doi:10.6165/tai.1995.40.1