%0 Journal Article
%A Ming-Chuan Wang
%T Separation of a Phloem Specific 42.5kDa Protein with Two-Dimensional Gel
%D 1997
%J Taiwania
%V 42
%N 3
%P 180-188
%U https://taiwania.ntu.edu.tw/abstract/365
%X Phloem exudates leaking from Luffa cylindrica fruits by a simple razor blade cutting were mixed with the extraction medium containing antioxidant and rapidly separated by 2-dimentional gel, i. E., native and sodium dodecylsulfate(SDS) polyacrylamide gel electrophoresis(PAGE). A major protein of 85kDa occupying 28.2% of total protein had 5 bands on denatured gel. One of them with molecular weight at 42.5kDa was complementally identified by reversed-phase high performance liauid chromatography and silver stained-band on SDS-gel.
%M doi:10.6165/tai.1997.42.180