%0 Journal Article %A Jen-Chih Chen %A Yih-Ming Chen and Kai-Wun Yeh %T Isolation and Characterization of New Sporamin Gene Members from Sweet Potato (Ipomoea batatas Lam.)(1) %D 1997 %J Taiwania %V 42 %N 1 %P 34-42 %U https://taiwania.ntu.edu.tw/abstract/81 %X Two full-length and two partial cDNAs encoding sporamin A have been isolated from sweet potato tuberous roots. Sequence comparisons show that they are very similar with 94-98% homology at nucleotide level, and 80-88% at protein level. All four cDNAs possess multiple alternate polyadenylation signals in the 3' untranslated region ( 3'-UTR). Genomic Southern blot analysis indicates the presence of a sporamin multigene family in sweet potato. High levels of sporamin mRNAs were detected in developing tuberous roots, but they disappeared at the sprout-germinating stage. Differential expression of these genes was obvious as their mRNAs were present specifically in developing roots, rarely in stems and not in leaves. %M doi:10.6165/tai.1997.42.34