%0 Journal Article
%A Shiau-Wei Lee and Shih-Tong Jeng 
%T Effects of GTP and ATP on the Transcription of T7 RNA Polymerase in the Linearized and Supercoiled DNA Templates
%D 2000
%J Taiwania
%V 45
%N 1
%P 1-12
%U https://taiwania.ntu.edu.tw/abstract/247
%X The effects of GTP and ATP on the transcription termination and transcript production of bacteriophage T7 RNA polymerase were analyzed at the thr attenuator in the supercoiled and linearized DNA templates. T7 RNA polymerase and the supercoiled or linearized DNA template with the thr attenuator were preincubated with GTP or ATP first, then transcription was started by the addition of all four nucleotides. The results indicated that the termination efficiency of T7 RNA polymerase at the thr attenuator is significantly higher in the supercoiled DNA template than in the linearized one. With the preincubation of GTP, T7 RNA polymerase generates 4-21 times more RNA transcript in the supercoiled than linearized DNA template. Also, in the supercoiled DNA, T7 transcription with the preincubation of GTP produces 3.6-5.0 times more RNA transcript than that without preincubation. This strongly suggests that the topology of DNA template influences the transcript production of T7 RNA polymerase. Moreover, GTP may change the conformation of T7 RNA polymerase in the complex of transcription initiation.
%M doi:10.6165/tai.2000.45(1).1