%0 Journal Article
%A Thing-Chien Tsay
%A Shieu-Yun Ma and Yung-Reui Chen
%T Cadmium-Binding Polypeptides in Cadmium-Treated Zinnia
%D 2002
%J Taiwania
%V 47
%N 1
%P 11-23
%U https://taiwania.ntu.edu.tw/abstract/307
%X Five-week-old seedlings of Zinnia elegans were hydroponically cultured in Hoagland's solution containing 45 M Cd2+ with or without one week of prior exposure to 10 M Cd2+. Profiles of HPLC indicated that plants previously exposed to 10 M Cd2+ developed more sulfurhydryl groups and glutathione than plants not exposed to that prior treatment. Individual peaks of sulfurhydryl groups displayed a prominent increase at 24 h, following by a gradual decrease to initial levels of sulfurhydryl after 96 h. Further isolation and purification of metal-binding complexes was done using DE 52, Sephadex, and HPLC chromatographic separations. Two fractions of the LMW complex contained more than 90% of the histidine-like substance in total amino acids. The HMW complex had five peaks in the HPLC profile. One major peak contained more than 85% of the histidine-like substance, whereas other peaks contained less than 0.1% of the histidine, along with phytochelatins with n number 3 and 4.
%M doi:10.6165/tai.2002.47(1).11