%0 Journal Article
%A S. John Britto
%A E. Natarajan and D. I. Arockiasamy
%T In Vitro Flowering and Shoot Multiplication from Nodal Explants of Ceropegia bulbosa Roxb. Var. bulbosa
%D 2003
%J Taiwania
%V 48
%N 2
%P 106-111
%U https://taiwania.ntu.edu.tw/abstract/36
%X A rapid micropropagation system was developed for Ceropegia bulbosa Roxb. Var. bulbosa. First five nodes of ~ 1.0 cm each, harvested from young healthy shoots from garden raised
plants were cultured on B5 medium supplemented with different concentrations of BA and AdS each in combination with 0.05 mg/L NAA. Multiple shoot formation of upto 12 shoots was observed within one week in presence of BAP (3 mg/L) and NAA (0.05 mg/L). Shoots were multiplied by subculture on the same medium. Shoots of 3-4 cm length were rooted in medium supplemented with 2 mg/L IBA. The rooted plantlets were hardened and successfully established in pots at 70% success rate. In vitro flowering was observed at 0.5 mg/L BA+1 mg/L GA3. Shoots transferred to the medium containing kinetin (0.05 mg/L) + IBA (2 mg/L) showed microtubers in 28 days while the shoots cultured in presence of 1 mg/L GA3 +0.5 mg/L of BA showed 76% flowering leading to seed production and 65% of the seeds germinated in successive generations.
%M doi:10.6165/tai.2003.48(2).106