%0 Journal Article
%A Hsiu-Hui Shih
%T A Polymerase Chain Reaction for Detecting Herpesvirus anguillae in Asymptomatic Eels
%D 2004
%J Taiwania
%V 49
%N 1
%P 1-6
%U https://taiwania.ntu.edu.tw/abstract/9
%X The present study describes a polymerase chain reaction (PCR) assay for detection of Herpesvirus anguillae (HVA) DNA. PCR primers (622F and 622R) were developed based on a
HindIII-1550 bp DNA fragment from HVA genomic library. Templates of HVA DNA yielded an evident amplification product showing the expected mobility of a 622-bp DNA fragment. The assay is rapid, sensitive and specifically detects HVA DNA including both the Taiwanese and the Japanese isolates. HVA persistent infections in asymptomatic farmed eels at Taiwan were revealed successfully in the present study.
%M doi:10.6165/tai.2004.49(1).1