%0 Journal Article
%A K. Shankarmurthy
%A V. Krishna
%A K. R. Maruthi and B. A. Rahiman
%T Rapid Adventitious Organogenesis from Leaf Segments of Embelia ribes Burm.－a Threatened Medicinal Plant
%D 2004
%J Taiwania
%V 49
%N 3
%P 194-200
%U https://taiwania.ntu.edu.tw/abstract/424
%X An in vitro protocol was developed for rapid multiplication of plantlets via direct organogenesis from the leaf segments of Embelia ribes Burm. (Myrsinaceae), a rare woody medicinal shrub under threat of being extinct. Adventitious shoots were organized directly from the margin of the lamina on medium supplemented with 2 to 4 mg/L 6-furfuryl amino purine (FAP) and 0.2 to 0.6 mg/L naphthalene acetic acid (NAA). The frequency of shoot bud production was the highest (mean of 33.6 ± 3.63 shoots per explants) at the concentration of 3 mg/L FAP and 0.4 mg/L NAA. Rooting of microshoots was also noticed on the same medium in a single-phase culture. A mean of 30 ± 1.05 root intact plantlets was recovered per explant. The rooted plantlets were well accomplished with a survival frequency of 96%. Moreover, there were no phenotypic differences observed between the in vitro regenerated and in vivo plants.
%M doi:10.6165/tai.2004.49(3).194