%0 Journal Article
%A Kumar Kamal Anand
%A Ravi Kumar Srivastava
%A Lal Babu Chaudhary and Arun Kumar Singh
%T Delimitation of Species of the Astragalus rhizanthus Complex (Fabaceae)
Using Molecular Markers RAPD, ISSR and DAMD
%D 2010
%J Taiwania
%V 55
%N 3
%P 197-207
%U https://taiwania.ntu.edu.tw/abstract/1136
%X Forty accessions belonging to four closely related species of the Astragalus rhizanthus complex (i. e., A. rhizanthus,
A. candolleanus, A. malacophyllus and A. pindreensis) were collected from different parts of the Indian Himalaya and analyzed
for intra- and inter-specific relationship using Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats
(ISSR) and Directed Amplification of Minisatellite DNA (DAMD). Eighteen RAPD, 15 ISSR and 6 DAMD primers were used to
study the genetic diversity between the species. A total of 449 bands for RAPD, 326 bands from ISSR and 179 bands from
DAMD were obtained. Nearly 98.44% of RAPD, 99.38% of ISSR and 98.32% of DAMD bands were polymorphic. Pair-wise
genetic similarity for a cumulative data was determined using Jaccard's similarity coefficient which varied from 0.23 to 0.82 and a
Neighbor-joining (NJ) dendrogram was generated. The genetic similarity from the data matrix estimated by Jaccard's coefficient
shows that all four species are distinct in accordance with our morphological findings as well as previous taxonomic treatment in
which these species have been treated distinctly. The highest degree of genetic similarity was observed within A. rhizanthus
followed by A. malacophyllus, A. candolleanus and A. pindreensis. This study has proved that these markers have the ability to
distinguish the closely allied species as well as analyze the genetic diversity within and between the species.
%M doi:10.6165/tai.2010.55(3).197