%0 Journal Article
%A Min-Yuan Ho
%A Wen-Chuan Chung
%A Hung-Chang Huang
%A Wen-Hsia Chung and Wen-Hsin Chung
%T Identification of Endophytic Fungi of Medicinal Herbs of Lauraceae and Rutaceae with Antimicrobial Property
%D 2012
%J Taiwania
%V 57
%N 3
%P 229-241
%U https://taiwania.ntu.edu.tw/abstract/1180
%X This study was conducted to determine taxonomical features and antimicrobial activities of 156 isolates of endophytic fungi collected from twigs of medicinal plants of Lauraceae (67 isolates) and Rutaceae (89 isolates) in central and northern Taiwan. The 156 isolates of fungi were classified into 35 genera in 19 families based on morphological characteristics of mycelia and asexual/sexual spores, as well as molecular phylogenetic analysis of rDNA LSU D1/D2 and ITS regions. The most
common endophytes were in the taxa of Colletotrichum, Guignardia, Hypoxylon, Nigrospora, Phomopsis and Xylaria, and the most common hosts were Citrus and Zanthoxylum of Rutaceae and Cinnamomum of Lauraceae. Molecular phylogenetic analysis showed that xylariaceous isolates could be separated into Xylaria and Hypoxylon groups based on rDNA of LSU D1/D2 and ITS regions. Four isolates of endophytic fungi including Lasmenia sp. isolate CB10, Ophioceras tenuisporum isolate CI02, Xylaria cubensis isolate LA04 and Cyanodermella sp. isolate TR09 were tested for antimicrobial activities using a dual culture method and Lasmenia sp. isolate CB10 and Cyanodermella sp. isolate TR09 showed better antimicrobial activity against 12 plant pathogens including 9 fungi and 3 bacteria. Spraying Chinese cabbage (Brassica rapa) plants with culture filtrates of the endophytic fungus Lasmenia sp. isolate CB10 significantly reduced severity of anthracnose of Chinese cabbage caused by Colletotrichum higginsianum under greenhouse conditions. This study suggests that the Lasmenia sp. isolate CB10 may be of potential for management of anthracnose
of Chinese cabbage.
%M doi:10.6165/tai.2012.57(3).229