TY -JOUR
AU -Paul Po-Chao Lin
TI -Enzymatic Hydrolysis of Cyclic Nucleoside Monophosphate in Barley Seedlings
PY -1973
DA -0000-00-00
JO -Taiwania
VL -18
IS -2
SP -109
EP -117
UR -https://taiwania.ntu.edu.tw/abstract/842
AB -The enzymatic hydrolysis of cyclic nucleotide monophosphate in barley seedlings (Hordeum vulgare L ., cucltivar Himalaya) was investigated. Multiple forms of enzyme activity toward adenosine-2', 3'-cyclic monophosphate are observed. At least one of these is capable of catalyzing the hydrolysis of adenosine-3', 5'-cyclic monophosphate. Adenosine is the sole product from the hydrolysis of cyclic AMP because of the presence of nucleotidase activity. Further studies on substrate specificity, sedimentation behavior, pH optimum metal ion effect, dithiothreitol effect and Km values suggest that the hydrolysis of cyclic nucleoside monphosphate may be due to a nonspecific acid phosphatase.
DO -10.6165/tai.1973.18.109