TY -JOUR AU -Shu-Mei Pan AU -Yu-Fen Huang AU -Yung-Reui Chen TI -Characterization and Localization of Acid Phosphatases in the Suspension-Cultured Cells of Nicotiana otophora L. PY -1987 DA -0000-00-00 JO -Taiwania VL -32 IS -1 SP -1 EP -10 UR -https://taiwania.ntu.edu.tw/abstract/556 AB -Active cells were harvested from tobacco cell suspension culture. The acid phosphatase of tobacco cells was mostly located in the cell wall and vacuole. Similar zymogram of acid phosphatase revealed in the gradient polyacrylamide gel was observed between the tobacco leaves and their induced-callus. Multiple acid phosphatases of tobacco suspension cells esperated by chromatography showed similar substrate specificities to most compounds tested. Zn2+ has differential inhibition on the multiple forms of acid phosphatase of tobacco cells. DO -10.6165/tai.1987.32.1