TY -JOUR
AU -Shih-Tong Jeng
TI -The Effects of Salts and Nucleoside Triphosphates on the Transcription Termination of Bacteriophage RNA Polymerases
PY -1995
DA -0000-00-00
JO -Taiwania
VL -40
IS -4
SP -403
EP -418
UR -https://taiwania.ntu.edu.tw/abstract/898
AB -The thr attenuator is a DNA element containing a G+C-rich dyad symmetry followed by a run of template poly (dA), and is reported to be a rho-independent terminator. The effects of the concentrations of salts, magnesium chloride, and nucleotide triphosphates on the transcription of bacteriophage T7, T3, and SP6 RNA polymerases were analyzed and compared with the nearly identical DNA templates with thr attenuator. Results indicate that in the low concentration of magnesium chloride or high amount of sodium chloride or potassium glutamate, termination by T7, T3, and SP6 RNA polymerases is enhanced. Also, inresponse to high NTP concentration the termination efficiencies of T7 and T3 RNA polymerases are enhanced and that of SP6 RNA polymerase remain constant.
DO -10.6165/tai.1995.40.403