TY -JOUR
AU -Ming-Chuan Wang
TI -Separation of a Phloem Specific 42.5kDa Protein with Two-Dimensional Gel
PY -1997
DA -0000-00-00
JO -Taiwania
VL -42
IS -3
SP -180
EP -188
UR -https://taiwania.ntu.edu.tw/abstract/365
AB -Phloem exudates leaking from Luffa cylindrica fruits by a simple razor blade cutting were mixed with the extraction medium containing antioxidant and rapidly separated by 2-dimentional gel, i. E., native and sodium dodecylsulfate(SDS) polyacrylamide gel electrophoresis(PAGE). A major protein of 85kDa occupying 28.2% of total protein had 5 bands on denatured gel. One of them with molecular weight at 42.5kDa was complementally identified by reversed-phase high performance liauid chromatography and silver stained-band on SDS-gel.
DO -10.6165/tai.1997.42.180