TY -JOUR AU -Jen-Chih Chen AU -Yih-Ming Chen and Kai-Wun Yeh TI -Isolation and Characterization of New Sporamin Gene Members from Sweet Potato (Ipomoea batatas Lam.)(1) PY -1997 DA -0000-00-00 JO -Taiwania VL -42 IS -1 SP -34 EP -42 UR -https://taiwania.ntu.edu.tw/abstract/81 AB -Two full-length and two partial cDNAs encoding sporamin A have been isolated from sweet potato tuberous roots. Sequence comparisons show that they are very similar with 94-98% homology at nucleotide level, and 80-88% at protein level. All four cDNAs possess multiple alternate polyadenylation signals in the 3' untranslated region ( 3'-UTR). Genomic Southern blot analysis indicates the presence of a sporamin multigene family in sweet potato. High levels of sporamin mRNAs were detected in developing tuberous roots, but they disappeared at the sprout-germinating stage. Differential expression of these genes was obvious as their mRNAs were present specifically in developing roots, rarely in stems and not in leaves. DO -10.6165/tai.1997.42.34