TY -JOUR
AU -Chi-Ying Huang
AU -Eng-Chiau Liau and Tei-Yei Kuo
TI -Effects of Salt-Stress on the Biosynthesis of Lipids in Chloroplast Membranes of Soybean Plant (1)
PY -1997
DA -0000-00-00
JO -Taiwania
VL -42
IS -1
SP -63
EP -72
UR -https://taiwania.ntu.edu.tw/abstract/84
AB - Soybean plants were grown under salt-stressed condition ( w = -1.6 Mpa) without or with previous acclimation at w = -1.2 Mpa. The chloroplasts that isolated from those salt-stressed plants were incubated with [14C] acetate. The chloroplast membranes were then subfractionated using linear density sucrose gradient centrifugations into outer envelope membrane (o. e. m.) and inner envelope membranes (i. e. m.). The acyl-CoA synthetase and UDP-galactose diacylglycerol galactosyltransferase activities in both envelope membranes were assayed. Oleic and palmitic acid were the major fatty acids in lipid of these two envelope membranes. Salt-stress reduced chloroplasts to synthesize oleic and palmitic acid, but acclimation caused chloroplasts to recover partially the levels of fatty acids biosynthetic activity. Oleic and palmaitic acid contents in lipid of o.e.m. increased from 6 to 19 %, and 4.6 to 17 % of the control levels if plants had acclimated previously to w = -1.2 Mpa. The concentrations of these two kinds of fatty acid in lipid of i.e.m., likely in o.e.m., were recovered from 1.8 to 45 %, and 4.6 to 17 % of the control ones. Over-all, lipid biosynthesis in i.e.m. of chloroplast were more sensitive to salt-stress treatment compared to o.e.m.. This suggested that activities of lipid biosynthesis in i. e. m. of chloroplast might have a regulatory mechanism for plants to adapt the salt-stressed environment.
DO -10.6165/tai.1997.42.63