TY -JOUR
AU -Li-Ming Chen and Kuo-Chieh Ho
TI -Characterization of a Phytoplasma-related Ribonuclease in Catharanthus roseus (L.) G. Don
PY -1997
DA -0000-00-00
JO -Taiwania
VL -42
IS -1
SP -8
EP -16
UR -https://taiwania.ntu.edu.tw/abstract/79
AB -A 6.8 kDa ribonuclease protein was found in the protein extract of the diseased periwinkle ( Catharanthus roseus (L.) G. Don ) infected by the phytoplasma of loofah witches' broom, but not in that of the healthy plants. This protein has an isoelectric point between 4 and 5. Further analyses revealed that it had a broad optimum pH of 3 to 8, and was sensitive to inhibition by CaCl2 (2 mM) and MgCl2 (2 mM) and less seneitive to ZnCl2 (50 M), but insensitive to EDTA (250 mM) and NaCl (5mM).
		In order to investigate if this Rnase could also be induced by other factors, the healthy periwinkle were given different environmental stress treatments including heat shock, drought, ethanol, and abscisic acid. None of these treatments induced the expression of this phytoplasma-related Rnase protein.
DO -10.6165/tai.1997.42.8