TY -JOUR
AU -Ling-Long Kuo-Huang and Shiang-Jiuun Chen
TI -Subcellular Localization of Calcium in the Crystal-Forming Sclereids of Nymphaea tetragona Georgi
PY -1999
DA -0000-00-00
JO -Taiwania
VL -44
IS -4
SP -520
EP -528
UR -https://taiwania.ntu.edu.tw/abstract/154
AB -By using a modified osmium tetroxide-potassium pyroantimonate (OTPA) precipitation technique, calcium ions were subcellularly localized in the crystal-forming sclereids of the leaf of Nymphaea tetragona Georgi. In young leaf laminae, the mesophyll parenchyma cells contained calcium antimonate precipitates in the vacuoles, mitochondria, nuclei, endoplasmic reticulum, dictyosome and the secretary vesicles. Besides, calcium precipitates were also associated with the plasmalemma and plasmodesmata. From the rudimentary mesophyll cells, the sclereid idioblasts originated sporadically. In these sclereid initial cells, many prismatic oxalate crystals bounded by the crystal sheaths were observed in the crystal chambers between the plasmalemma and primary cell wall. The calcium antimonate precipitates were densely presented on the crystal sheaths, however, only few calcium precipitates were found in the central vacuoles of the sclereid initial cells. After the formation of crystals, the secondary wall was deposited and then the crystals were embedded between the primary and secondary cell walls. In the mature sclereid, calcium antimonate precipitates were obviously associated with the crystal sheaths, primary cell wall, plasmalemma, and also accumulated in the central vacuole.
DO -10.6165/tai.1999.44(4).520