TY -JOUR
AU -T. Anuradha and T. Pullaiah
TI -Effect of Hormones on the Organogenesis and the Somatic Embryogenesis of an Endangered Tropical Forest Tree - Hildegardia populifolia (Roxb.) Schott. & Endl.
PY -2001
DA -0000-00-00
JO -Taiwania
VL -46
IS -1
SP -62
EP -74
UR -https://taiwania.ntu.edu.tw/abstract/279
AB -Nodal explants from aseptic seedlings of Hildergardia populifolia cultured on Murashige and Skoog’s (MS) medium supplemented with 2 mgL-1 N6-benzylaminopurine (BAP) generated very few shoots over a period of 30 days. Shoots were rooted in vitro on 1/2 strength MS macro salts containing 2 mgL-1 naphthalene acetic acid (NAA). A significant achievement in the present investigation was the production of somatic embryos from immature zygotic embryo explants exposed to MS medium supplemented with NAA 3 mgL-1 and casein hydrolysate (CH) 1000 mgL-1 over a period of 60 days. Subsequent withdrawl of NAA and CH from the medium resulted in the maturation and growth of embryos into plantlets on MS medium. The plantlets were transferred to sand: manure: soil mixture (1:1:1) where they showed no visual abnormalities in morphological characters.
DO -10.6165/tai.2001.46(1).62