TY -JOUR
AU -M. Y. Sreenivasa
AU -Regina Sharmila Dass
AU -A. P. Charith Raj and G. R. Janardhana
TI -Molecular Detection of Fumonisin Producing Fusarium Species of Freshly Harvested Maize Kernels Using Polymerase Chain
Reaction (PCR)
PY -2006
DA -0000-00-00
JO -Taiwania
VL -51
IS -4
SP -251
EP -257
UR -https://taiwania.ntu.edu.tw/abstract/511
AB -Fumonisins are a new class of toxic secondary metabolites produced by Fusarium
species namely Fusarium moniliforme and Fusarium proliferatum that have recently come under
intense scientific investigations. Thirty-two Fusarium isolates namely, Fusarium moniliforme (12
isolates), Fusarium proliferatum (5 isolates), Fusarium anthophilum (4 isolates), Fusarium oxysporum
(3 isolates), Fusarium sporotrichioides (3 isolates), Fusarium pallidoroseum (3 isolates) and Fusarium
solani (2 isolates), other four isolates of Aspergillus flavus (2 isolates) and Alternaria alternata (2
isolates), isolated from freshly harvested maize kernels were subjected to PCR analysis. The objective
of this study was to develop a PCR-based detection kit for detection of fumonisin-producing Fusarium
species. One set of primers based on the conserved ITS region of the genus Fusarium was used to
differentiate the genus Fusarium from other fungal genera such as Aspergillus and Alternaria. The
other set of primers specific to fumonisin producing ‘FUM 1gene’ region of Fusarium was used to
differentiate the fumonisin producing Fusarium species from non-fumonisin producers. Results of the
study revealed that PCR-based technique could be used not only to differentiate the Fusarium species
from other genera of fungi but also to identify fumonisin-producing Fusarium moniliforme, F.
Proliferatum and F. anthophilum.
DO -10.6165/tai.2006.51(4).251