TY -JOUR
AU -Masaya Yamamoto
AU -Daiki Takahashi
AU -Chih-Chieh Yu
AU -Hiroaki Setoguchi
TI -Development and characterization of EST-SSR markers in creeping mazus (Mazus miquelii), and cross-amplification in five related species
PY -2020
DA -2020-04-18
JO -Taiwania
VL -65
IS -2
SP -249
EP -252
UR -https://taiwania.ntu.edu.tw/abstract/1680
AB -Simple sequence repeat (SSR) markers were developed from expressed sequence tags (ESTs) for Mazus miquelii (Mazaceae), one of the most widespread species of the genus found in mainland China to Japan and North America, with the goal of elucidating the hidden genetic diversity and pollination ecology of the species. Of the initial 48 EST-SSR markers designed based on transcriptome data, 36 loci were successfully amplified, 16 of which were polymorphic. Polymorphisms for these markers were tested on 72 individuals from three populations in Japan. Two to eleven alleles per locus were detected, and the levels of observed and expected heterozygosity ranged from 0.181 to 0.708 and 0.154 to 0.715, respectively. Most loci were amplified successfully in five related Japanese and Taiwanese species. These markers will empower ecological and evolutionary studies in the creeping mazus and facilitate the disentanglement of phylogenetic relationships with related species.
DO -10.6165/tai.2020.65.249