Research Paper

Genetic diversity of Cyclina sinensis (Veneridae): Resource management in Taiwan

Kui-Ching Hsu, Hsin-Ju Wu, Po-Hsun Kuo, Yuh-Wen Chiu

Published on: 15 April 2021

Page: 165 - 173

DOI: 10.6165/tai.2021.66.165

PDF Download pdf

2021 vol.66 no.2 pp.165-173



Allendorf, F.W. and S.R. Phelps. 1980. Loss of genetic variation in a hatchery stock of cutthroat trout. Trans. Am. Fish. Soc. 109(5): 537–543.
DOI: 10.1577/1548-8659(1980)109<537:LOGVIA>2.0.CO;2View ArticleGoogle Scholar

Bandelt, H.J., P. Forster and A. R?hl. 1999. Median-joining networks for inferring intraspecific phylogenies. Mol. Biol. Evol. 16(1): 37–48.
DOI: 10.1093/oxfordjournals.molbev.a026036View ArticleGoogle Scholar

Beerli, P. 2016. MIGRATE-N 4.4.3. https:

Cheang, C.C., K.H. Chu and P.O. Ang Jr. 2010. Phylogeography of the marine macroalga Sargassum hemiphyllum (Phaeophyceae, Heterokontophyta) in northwestern Pacific. Mol. Ecol. 19(14): 2933–2948.
DOI: 10.1111/j.1365-294X.2010.04685.xView ArticleGoogle Scholar

Chen, H.Y and Y.D. Ho. 2001. Production of hard clam seed. In G. F. Liu (Ed.), Breeding and aquaculture techniques for fishes and clams in southwestern Taiwan, (pp. 81–109). Yunlin, Taiwan: Taishi Branch, Fisheries Research Institute, Council of Agriculture (in Chinese).

Chen, J., Q. Li, L. Kong and H. Yu. 2011. How DNA barcodes complement taxonomy and explore species diversity: the case study of a poorly understood marine fauna. PLOS one 6(6): e21326.
DOI: 10.1371/journal.pone.0021326View ArticleGoogle Scholar

Chen, L.C. 1990. Aquaculture in Taiwan. Oxford, UK: Blackwell Scientific Publications.

Chiu, Y.W., H. Bor, P.H. Kuo, K.C. Hsu, M.S. Tan, W.K. Wang and H.D. Lin. 2017. Origins of Semisulcospira libertina (Gastropoda: Semisulcospiridae) in Taiwan. Mitochondrial DNA. A 28(4): 518–525.
DOI: 10.3109/24701394.2016.1149823View ArticleGoogle Scholar

Chu, T.J., D. Wang, H.L. Huang, F.J. Lin and T.D. Tzeng. 2012. Population structure and historical demography of the whiskered velvet shrimp (Metapenaeopsis barbata) off China and Taiwan inferred from the mitochondrial control region. Zool. Stud. 51: 99–107.

Council of Agriculture. 2020. Artificial breeding of Cyclina sinensis succeeded.

Darriba, D., G.L. Taboada, R. Doallo and D. Posada. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nat. Methods 9(8): 772.
DOI: 10.1038/nmeth.2109View ArticleGoogle Scholar

Dong, P., G. Ma, L. Chang, Y. Zhu and X. Tian. 2016. The complete mitochondrial genome of Cyclina sinensis (Veneroida:Veneridae). Mitochondrial DNA. B 1(1): 173–174.
DOI: 10.1080/23802359.2016.1149786View ArticleGoogle Scholar

Dong, Y.W., H.S. Wang, G.D. Han, C.H. Ke, X. Zhan, T. Nakano and G.A. Williama. 2012. The impact of Yangtze River discharge, ocean currents and historical events on the biogeographic pattern of Cellana toreuma along the China coast. PLOS one 7(4): e36178.
DOI: 10.1371/journal.pone.0036178View ArticleGoogle Scholar

Drummond, A.J., A. Rambau and M. Suchard. 2013. BEAST 1.8.0.

Dupanloup, I., S. Schneider and L. Excoffier. 2002. A simulated annealing approach to define the genetic structure of populations. Mol. Ecol. 11(12): 2571–2581.
DOI: 10.1046/j.1365-294X.2002.01650.xView ArticleGoogle Scholar

Excoffier, L. and H.E. Lischer. 2010. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol. Ecol. Resour. 10(3): 564–567.
DOI: 10.1111/j.1755-0998.2010.02847.xView ArticleGoogle Scholar

Folmer, O., M. Black, W. Hoeh, R. Lutz and R. Vrijenhoek. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotech. 3(5): 294–299.

Fu, Y.X. 1997. Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147(2): 915–925.
DOI: 10.1093/genetics/147.2.915View ArticleGoogle Scholar

Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Sym. 41: 95–98.

Han, C.C., K.C. Hsu, L.S. Fang, I.M. Chang and H.D. Lin. 2019. Geographical and temporal origins of Neocaridina species (Decapoda: Caridea: Atyidae) in Taiwan. BMC Genetics 20(1): 86.
DOI: 10.1186/s12863-019-0788-yView ArticleGoogle Scholar

He, L., T. Mukai, C.K. Hou, Q. Ma and J. Zhang. 2015. Biogeographical role of the Kuroshio Current in the amphibious mudskipper Periophthalmus modestus indicated by mitochondrial DNA data. Sci. Rep. 5(1): 15645.
DOI: 10.1038/srep15645View ArticleGoogle Scholar

Hellberg, M.E. and V.D. Vacquie. 1999. Rapid evolution of fertilization selectivity and lysin cDNA sequences in teguline gastropods. Mol. Biol. Evol. 16(6): 839–848.
DOI: 10.1093/oxfordjournals.molbev.a026168View ArticleGoogle Scholar

Hsu, K.C., H. Bor, H.D. Lin, P.H. Kuo, M.S. Tan and Y.W. Chiu. 2014. Mitochondrial DNA phylogeography of Semisulcospira libertine (Gastropoda: Cerithioidea: Pleuroceridae): implications the history of landform changes in Taiwan. Mol. Biol. Rep. 41(6): 3733–3743.
DOI: 10.1007/s11033-014-3238-yView ArticleGoogle Scholar

Huang, H.T., C.N. Pao, T.Y. Liao and L.L. Liu. 2020. Low genetic diversity of cultivated spotted hard clam (Meretrix petechialis) in Taiwan. Aquac Res. 51(7): 2962– 2972.
DOI: 10.1111/are.14634View ArticleGoogle Scholar

Jiang, X., Z. Ren, B. Zhao, S. Zhou, X. Ying and Y. Tang. 2020. Ameliorating Effect of Pentadecapeptide Derived from Cyclina sinensis on Cyclophosphamide-Induced Nephrotoxicity. Mar. Drugs 18(9): 462
DOI: 10.3390/md18090462View ArticleGoogle Scholar

Jiang, Y., F. Chai, Z. Wan, X. Zhang and H. Hong. 2011 Characteristics and mechanisms of the upwelling in the southern Taiwan Strait: a three-dimensional numerical model study. J. Oceanogr. 67(6): 699–708.
DOI: 10.1007/s10872-011-0080-xView ArticleGoogle Scholar

Kumar, S., G. Steecher, M. Li, C. Knyaz and K. Tamura. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35(6): 1547–1549.
DOI: 10.1093/molbev/msy096View ArticleGoogle Scholar

Li, W., S. Ye, Z. Zhang, J. Tang, H. Jin, F. Huang, Z. Yang, Y. Tang, Y. Chan, G. Ding and F. Yu. 2019. Purification and characterization of a novel pentadecapeptide from protein hydrolysates of Cyclina sinensis and its immunomodulatory effects on RAW264.7 cells. Mar. drugs 17(1): 30.
DOI: 10.3390/md17010030View ArticleGoogle Scholar

Li, X., Z. Dong, B. Yan, H. Cheng, X. Meng, H. Shen and J. Li. 2010. Analysis and evaluation of nutritional components in Cyclina sinensis and Meretrix meretrix. Food Sci. 31(3-4): 366–370 (in Chinese).

Liao, H.R., H.S. Yu and C.C. Su. 2008. Morphology and sedimentation of sand bodies in the tidal shelf sea of eastern Taiwan Strait. Mar. Geol. 248(3-4): 161–178.
DOI: 10.1016/j.margeo.2007.10.013View ArticleGoogle Scholar

Librado, P. and J. Rozas. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25(11): 1451–2.
DOI: 10.1093/bioinformatics/btp187View ArticleGoogle Scholar

Liu, J., Q. Li, L.F. Kong and X.D. Zheng. 2011. Cryptic diversity in the pen shell Atrina pectinata (Bivalvia: Pinnidae): high divergence and hybridization revealed by molecular and morphological data. Mol. Ecol. 20(20): 4332–4345.
DOI: 10.1111/j.1365-294X.2011.05275.xView ArticleGoogle Scholar

Liu, J., T.X. Gao, S. Wu and Y.A.P. Zhang. 2007. Pleistocene isolation in the northwestern Pacific marginal seas and limited dispersal in a marine fish, Chelon haematocheilus (Temminck & Schlegel, 1845). Mol. Ecol. 16(2): 275–288.
DOI: 10.1111/j.1365-294X.2006.03140.xView ArticleGoogle Scholar

Marko, P.B. 2002. Fossil calibration of molecular clocks and the divergence times of geminate species pairs separated by the Isthmus of Panama. Mol. Biol. Evol. 19(11): 2005–2021.
DOI: 10.1093/oxfordjournals.molbev.a004024View ArticleGoogle Scholar

Nei, M. 1987. Molecular Evolutionary Genetics. New York: Columbia University.
DOI: 10.7312/nei-92038View Article

Nei, M. and F. Tajima. 1983. Maximum likelihood estimation of the number of nucleotide substitutions from restriction sites data. Genetics 105(1): 207–217.
DOI: 10.1093/genetics/105.1.207View ArticleGoogle Scholar

Ni, G., Q. Li, L. Kong and H. Yu. 2014. Comparative Phylogeography in marginal seas of the northwestern Pacific. Mol. Ecol. 23(3): 534–548.
DOI: 10.1111/mec.12620View ArticleGoogle Scholar

Ni, G., Q. Li, L. Kong and X. Zheng. 2012. Phylogeography of bivalve Cyclina sinensis: testing the historical glaciations and Changjiang River outflow hypotheses in Northwestern Pacific. PLOS one 7(11): e49487.
DOI: 10.1371/journal.pone.0049487View ArticleGoogle Scholar

Othmen, A.B., M. Abhary, T. Deli, Z. Ouanes, N. Alhuwautu, N. Dimassi and L. Mansour. 2020. Lack of mitochondrial genetic structure in the endangered giant clam populations of Tridacna maxima (Bivalvia: Cardiidae: Tridacninae) across the Saudi Arabian coast. Acta Oceanol. Sin. 39(2): 28–37.
DOI: 10.1007/s13131-020-1547-7View ArticleGoogle Scholar

Pan, B.P., L.S. Song, W.J. Bu and J.S. Sun. 2005. Studied on genetic diversity and differentiation between two allopatric populations of Cyclina sinensis. Acta Hydrobiol. Sin. 29: 372–378.

Rambaut, A., A.J. Drummond and M. Suchard. 2013. Tracer v1.6. Accessed 11 Dec.

Ramos-Onsins, S.E. and J. Rozas. 2002. Statistical properties of new neutrality tests against population growth. Mol. Biol. Evol. 19(12): 2092–2100.
DOI: 10.1093/oxfordjournals.molbev.a004034View ArticleGoogle Scholar

Sanna, D., T. Lai, P. Cossu, F. Scarpa, G.L. Dedola, B. Cristo, P. Francalacci, M. Curini-Galletti, L. Mura, N. Fois, F. Maltagliati and M. Casu. 2017. Cytochrome c oxidase subunit I variability in Ruditapes decussatus (Veneridae) from the western Mediterranean. The European Zool. J. 84(1): 554–565.
DOI: 10.1080/24750263.2017.1395914View ArticleGoogle Scholar

Shen, K.N., B.W. Jamandre, C.C. Hsu, W.N. Tzeng and J.D. Durand. 2011. Plio-Pleistocene sea level and temperature fluctuations in the northwestern Pacific promoted speciation in the globally-distributed flathead mullet Mugil cephalus. BMC Evol. Biol. 11(1): 83.
DOI: 10.1186/1471-2148-11-83View ArticleGoogle Scholar

Tajima, F. 1989. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123(3): 585–595.
DOI: 10.1093/genetics/123.3.585View ArticleGoogle Scholar

Templeton, A.R. 1993. The “Eve” hypotheses: a genetic critique and reanalysis. Am. Anthropol. 95(1): 51–72.
DOI: 10.1525/aa.1993.95.1.02a00030View ArticleGoogle Scholar

Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25(24): 4876–4882.
DOI: 10.1093/nar/25.24.4876View ArticleGoogle Scholar

Wang, R.C. and Z.P. Wang. 2008. Science of marine shellfish culture China. Qingdao: China Ocean University Press (in Chinese).

Wang, R.C., Z.P. Wang and J.Z. Zhang. 1993. Aquaculture of marine mollusks. Qingdao: China Ocean University Press (in Chinese).

Xu, J., T.Y. Chan, L.M. Tsang and K.H. Chu. 2009. Phylogeography of the mitten crab Eriocheir sensu stricto in East Asia: Pleistocene isolation, population expansion and secondary contact. Mol. Phylogenet. Evol. 52(1): 45–56.
DOI: 10.1016/j.ympev.2009.02.007View ArticleGoogle Scholar

Xue, D.X., H.Y. Wang, T. Zhang and J.X. Liu. 2014. Population genetic structure and demographic history of Atrina pectinata based on mitochondrial DNA and microsatellite markers. PLOS one 9(5): e95436.
DOI: 10.1371/journal.pone.0095436View ArticleGoogle Scholar

Yan, Y.R., K.C. Hsu, M.R. Yi, B. Li, W.K. Wang, B. Kang and H.D. Lin. 2020. Cryptic diversity of the spotted scat Scatophagus argus (Perciformes: Scatophagidae) in South China Sea: pre- or post-production isolation. Mar. Freshwater Res. 71(12): 1640–1650.
DOI: 10.1071/MF19337View ArticleGoogle Scholar

Yang, J.Q., K.C. Hsu, Z.Z. Liu, L.W. Su, P.H. Kuo, W.Q. Tang, Z.C. Zhou, D. Liu, B.L. Bao and H.D. Lin. 2016. The population history of Garra orientalis (Teleostei: Cyprinidae) using mitochondrial DNA and microsatellite data with approximate Bayesian computation. BMC Evol. Biol. 16(1): 73.
DOI: 10.1186/s12862-016-0645-9View ArticleGoogle Scholar

Yang, X., H. Ji, Y. Yu, J. Guan and Y. Sun. 2004. Preliminary study of the juvenile nursery of Cyclina sinensis. J. Shanghai Fish Univ. 13(1-3): 213–217 (in Chinese).

Yu, Y.S. and X.D. Zheng. 1995. The morphology and structure of the venus clam. Marine Fishery (in Chinese).

Zeng, Z.N. and F.X. Li. 1991. The study of reproductive cycle of Cyclina sinensis. Tropic Oceanology 10(4): 86–93.

Zhao, Y., Q. Li, L. Kong and Y. Mao. 2009. Genetic and morphological variation in the venus clam Cyclina sinensis along the coast of China. Hydrobiologia 635(1): 227–235.
DOI: 10.1007/s10750-009-9916-4View ArticleGoogle Scholar