Research Paper

Genetic diversity of Begonia versicolor (Begoniaceae), a narrow endemic species in southeast Yunnan of China

Chun Li, Jun-Lin Chen, Yong-Yong Li and Dai-Ke Tian

Published on: 12 March 2018

Page: 49 - 53

DOI: 10.6165/tai.2018.63.49

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    Abstract

    Begonia versicolor Irmscher, a narrow endemic Begonia species in southeast Yunnan of China, is a wonderful ornamental plant with huge diversity in colored foliage. To investigate its variations, the genetic diversity and population structure were studied based on 56 individuals sampled from four localities using 12 polymorphic microsatellite loci transferred from other species of Begonia. The results showed a relatively low level of genetic diversity in B. versicolor comparing with other species of Begonia using microsatellite. Positive inbreeding coefficient (FIS) values were found in three populations (SWC, XPZ and DSD). AMOVA analysis indicated that genetic variations occurred mainly within populations (55.9%) rather than among populations (9.7%) and among groups (34.4%). Four populations were grouped into two clusters based on STRUCTURE. AMOVA and STRUCTURE analysis showed a high level and significant genetic differentiation in the populations of B. versicolor. Based on its genetic status and rarity in the wild, the sustainable in-situ and ex-situ conservation strategies should be urgently carried out to protect this species with high horticultural and scientific values.

    Keyword: Begonia versicolor, Conservation strategies, Genetics diversity, Population structure, Yunnan

    Literature Cited

    Agren, J. and D.W. Schemske. 1991. Pollination by deceit in a neotropical monoecious herb, Begonia involucrata. Biotropica. 23(3): 235-241.
    DOI: 10.2307/2388200View Article Google Scholar

    Aitawade, M.M. and S. Yadav. 2012. Taxonomic status of Begonia aliciae (Begoniaceae). Rheedea. 22: 111-115.

    Chan, Y. C., L.T. Lee and S. Lee. 2014. Novel microsatellite markers for Begonia maxwelliana and transferability to 23 Begonia species of Peninsular Malaysia. Biochem. Syst. Ecol. 57: 159-163.
    DOI: 10.1016/j.bse.2014.08.004View Article Google Scholar

    De Lange, A. and F. Bouman. 1999. Seed micromorphology of neotropical begonias. Smiths. Contrib. Bot. 90: 1-49.
    DOI: 10.5479/si.0081024X.90View Article Google Scholar

    Dewitte, A., A.D. Twyford, D.C. Thomas, C.A. Kidner and J.V. Van Huylenbroeck. 2011. The origin of diversity in Begonia: genome dynamism, population processes and phylogenetic patterns. In: Christian, W., K. Janice and G. Hans-peter. The Dynamical Processes of Biodiversity - Case Studies of Evolution and Spatial Distribution, InTech Press, Rijeka, Croacia. 1: 20-29.
    DOI: 10.5772/1830View Article

    Earl, D.A. and B.M. vonHoldt. 2012. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv. Genet. Resour. 4(2): 359-361.
    DOI: 10.1007/s12686-011-9548-7View Article Google Scholar

    Evanno, G., S. Regnaut and J. Goudet. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol. Ecol. 14(8): 2611-2620.
    DOI: 10.1111/j.1365-294X.2005.02553.xView Article Google Scholar

    Excoffier, L. and H.E. Lischer. 2010. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol. Ecol. Resour. 10(3): 564-567.
    DOI: 10.1111/j.1755-0998.2010.02847.xView Article Google Scholar

    Francisco-Ortega, J., A. Santos-Guerra, S.C. Kim and D.J. Crawford. 2000. Plant genetic diversity in the Canary Islands: a conservation perspective. Am. J. Bot. 87(7): 909-919.
    DOI: 10.2307/2656988View Article Google Scholar

    Frankham, R., J.D. Ballou and D.A. Briscoe. 2002. Introduction to Conservation Genetics, Cambridge Univ. Press, Cambridge, UK. 227-253pp.
    DOI: 10.1017/CBO9780511808999View Article

    Goudet, J. 2001. FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9. 3). Available at: http://www2.unil.ch/popgen/softwares/fstat.htm (Accessed: 24 May 2017)

    Gu, C.-Z., C.-I. Peng and N.J. Turland. 2007. Begoniaceae. In: Wu, Z.-Y., P.H. Raven and D.-Y. Hong. Flora of China, Science Press, Beijing, China. 202pp.

    Gupta, P.K. and R. Varshney. 2000. The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica. 113(3): 163-185.
    DOI: 10.1023/A:1003910819967View Article

    Hamrick, J.L. and M.J.W. Godt. 1989. Allozyme diversity in plant species. In: Brown, A.H.D., M.T. Clegg, A.L. Kahler and B.S. Weir. Plant Population Genetics, Breeding and Genetic Resources. Sinauer, Sunderland, Massachusetts, UK. pp. 43-63.

    Hoover, W.S., C. Karegeannes, H. Wiriadinata and J.M. Hunter. 2004. Notes on the geography of South-East Asian Begonia and species diversity in montane forests. Telopea. 10: 749-764.

    Hughes, M. and P. Hollingsworth. 2008. Population genetic divergence corresponds with species-level biodiversity patterns in the large genus Begonia. Mol. Ecol. 17(11): 2643-2651.
    DOI: 10.1111/j.1365-294X.2008.03788.xView Article Google Scholar

    Hughes, M., P. Hollingsworth, and A. Miller. 2003. Population genetic structure in the endemic Begonia of the Socotra archipelago. Biol. Conserv. 113(2): 277-284.
    DOI: 10.1016/S0006-3207(02)00375-0View Article Google Scholar

    Hughes, M., P. Hollingsworth and J. Squirrell. 2002a. Isolation of polymorphic microsatellite markers for Begonia sutherlandii Hook. f. Mol. Ecol. Notes. 2(2): 185-186.
    DOI: 10.1046/j.1471-8286.2002.00201.xView Article Google Scholar

    Hughes, M., J. Russell and P. Hollingsworth. 2002b. Polymorphic microsatellite markers for the Socotran endemic herb Begonia socotrana. Mol. Ecol. Notes. 2(2): 159-160.
    DOI: 10.1046/j.1471-8286.2002.00185.xView Article Google Scholar

    Hughes, M., R.R. Rubite, P. Blanc, K.-F. Chung and C.-I Peng. 2015. The Miocene to Pleistocene colonization of the Philippine archipelago by Begonia sect. Baryandra (Begoniaceae). Am. J. Bot. 102(5): 1-12.
    DOI: 10.3732/ajb.1400428View Article Google Scholar

    Kiew, R., J. Sang, R. Repin and J.A. Ahmad. 2015. A Guide to Begonias of Borneo. Natural History Publications, Borneo, Malaysia. 1pp.

    Li, C., D.-K. Tian, X.-P. Li and N.-F Fu. 2015. Morphological and molecular identification of natural hybridization between Begonia hemsleyana and B. macrotoma. Sci. Hortic-Amsterda. 192: 357-360.
    DOI: 10.1016/j.scienta.2015.06.031View Article Google Scholar

    Li, Y.C., A.B. Korol, T. Fahima and E. Nevo. 2004. Microsatellites within Genes: Structure, Function, and Evolution. Mol. Bio. Evol. 21(6): 991-1007.
    DOI: 10.1093/molbev/msh073View Article Google Scholar

    Martin, M.A., C. Mattioni, M. Cherubini, D. Taurchini and F. Villani. 2010. Genetic diversity in European chestnut populations by means of genomic and genic microsatellite markers. Tree Genet. Genomes. 6(5): 735-744.
    DOI: 10.1007/s11295-010-0287-9View Article Google Scholar

    Matolweni, L.O., K. Balkwill and T. McLellan. 2000. Genetic diversity and gene flow in the morphologically variable, rare endemics Begonia dregei and Begonia homonyma (Begoniaceae). Am. J. Bot. 87(3): 431-439.
    DOI: 10.2307/2656639View Article Google Scholar

    Morgante, M. and A. Olivieri. 1993. PCR-amplified microsatellites as markers in plant genetics. Plant J. 3(1): 175-182.
    DOI: 10.1046/j.1365-313X.1993.t01-9-00999.xView Article Google Scholar

    Nakamura, K., C.J. Huang, R.R. Rubite, W.C. Leong, Y. Kono, H.A. Yang and C.-I Peng. 2012. Isolation of compound microsatellite markers in Begonia fenicis (Begoniaceae) endemic to East and Southeast Asian islands. Am. J. Bot. 99(1): e20-e23.
    DOI: 10.3732/ajb.1100297View Article Google Scholar

    Peakall, R. and P.E. Smouse. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Notes. 6(1): 288-295.
    DOI: 10.1111/j.1471-8286.2005.01155.xView Article Google Scholar

    Pritchard, J.K., M. Stephens and P. Donnelly. 2000. Inference of population structure using multilocus genotype data. Genetics. 155: 945-959.
    DOI: 10.1111/j.1471-8286.2007.01758.xView Article Google Scholar

    Rao, V.R. and T. Hodgkin. 2002. Genetic diversity and conservation and utilization of plant genetic resources. Plant Cell Tiss. Org. 68: 1-19.

    Schaal, B.A., W.J. Leverich and S.H. Rogstad. 1991. Comparison of methods for assessing genetic variation in plant conservation biology. Genet. Conserv. Rare Plants. 123-134.

    Tebbitt, M.C. 2005. Begonias: Cultivation, Identification, and Natural History. Timber Press, Portland.

    Thomas, D.C. 2010. Phylogenetics and historical biogeography of Southeast Asian Begonia L. (Begoniaceae). Doctoral thesis, University of Glasgow.

    Tseng, Y.-H., H.-Y. Huang, W.-B. Xu, H.-A. Yang, Y. Liu, C.-I Peng and K.-F. Chung. 2017. Development and characterization of EST-SSR markers for Begonia luzhaiensis (Begoniaceae). Appl. Plant Sci. 5(5): 1700024.
    DOI: 10.3732/apps.1700024View Article Google Scholar

    Twyford, A.D., R.A. Ennos and C.A. Kidner. 2013. Development and characterization of microsatellite markers for central American Begonia Sect. Gireoudia (Begoniaceae). Appl. Plant Sci. 1(5): 429-438.
    DOI: 10.3732/apps.1200499View Article Google Scholar

    Twyford, A.D., C. Kidner and R.A. Ennos. 2014. Genetic differentiation and species cohesion in two widespread Central American Begonia species. Heredity. 112(4): 382-390.
    DOI: 10.1038/hdy.2013.116View Article Google Scholar

    Twyford, A.D., C. Kidner and R.A. Ennos. 2015. Maintenance of species boundaries in a Neotropical radiation of Begonia. Mol. Ecol. 24(19): 4982-4993.
    DOI: 10.1111/mec.13355View Article Google Scholar

    Van Oosterhout, C., W.F. Hutchinson, D.P. Wills and P. Shipley. 2004. MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol. Ecol. Notes 4(3): 535-538.
    DOI: 10.1111/j.1471-8286.2004.00684.xView Article Google Scholar

    Waples, R.S. and C. Do. 2008. Idne: a program for estimating effective population size from data on linkage disequilibrium. Mol. Ecol. Resour. 8(4): 753-756.
    DOI: 10.1111/j.1755-0998.2007.02061.xView Article Google Scholar

    Wiesner, I. and D. Wiesnerova. 2008. Sequence-characterized markers from Begonia

    Zane, L., L. Bargelloni and T. Patarnello. 2002. Strategies for microsatellite isolation: a review. Mol. Ecol. 11(1): 1-16.
    DOI: 10.1046/j.0962-1083.2001.01418.xView Article Google Scholar