Research Paper

Applicability of the microbial inoculation method in the study on litter decomposition

Wenxing Zhou, Siran Zhang, Lijie Wang, Hongyan Hui, Xiaoxi Zhang

Published on: 22 August 2021

Page: 364 - 373

DOI: 10.6165/tai.2021.66.364

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    Abstract

    The decomposition of litter is one of the core links of the material cycling of ecosystem, studying this process is helpful for understanding the indirect effects of environmental alterations on the stability of ecosystems. In the traditional method litter is placed into litterbags which are placed outdoor on or in soil. Since under these conditions it is very difficult to analyze the parameters involved in litter decomposition separately, these experiments are also done indoor under controlled conditions. Inoculating the litter with soil or phyllosphere microorganisms and preventing any other interaction of the litter samples with the surrounding soil is an emerging method in the indoor investigation of plant litter decomposition, in order to experimentally minimize the impacts of invading soil constituents on litter decomposition parameters. However, few parallel-experiments were conducted to compare this inoculation method with the traditional litterbag method. Hence, it is unclear whether the inoculation method, when compared to the litterbag method, would cause artifacts with respect to the decomposition rate and microbial communities under controlled indoor conditions. In the present study, litters of Pinus tabuliformis, Salix babylonica and mixed litter from a Robinia pseudoacacia forest, representing litters with different decomposabilities, were chosen for a comparative experiment. Each type of litter was incubated under controlled indoor conditions for six months using the litterbag method and soil microorganism inoculation method. The decomposition rates and the litter fungal communities of the same litters were compared. Using microbial inoculation method, the decomposition rates exhibited no significant differences relative to those observed in the litterbag method. More importantly, the disturbance by biotic and abiotic factors from external soil on the fungal community in the samples could be excluded.

    Keyword: Fungal communities, litter decomposition, litterbag method, microbial inoculation method

    Literature Cited

    Asplund, J., H. Kauserud, S. Bokhorst, M.H. Lie, M. Ohlson and L. Nybakken. 2018. Fungal communities influence decomposition rates of plant litter from two dominant tree species. Fungal. Ecol. 32: 1–8.
    DOI: 10.1016/j.funeco.2017.11.003View Article Google Scholar

    Bani, A., S. Pioli, M. Ventura, P. Panzacchi, L. Borruso, R. Tognetti, G. Tonon and L. Brusetti. 2018. The role of microbial community in the decomposition of leaf litter and deadwood. Appl. Soil Ecol. 126: 75–86.
    DOI: 10.1016/j.apsoil.2018.02.017View Article Google Scholar

    Bao, S. 2000. Soil agro-chemistrical analysis. China Agriculture Press, Beijing. B?rlocher, F., M.O. Gessner and M.O.S. Gra?a. 2020. Methods to study litter decomposition: A Practical Guide. Berlin: Springer International Publishing.

    Berg, B. and C. MCclaugherty. 2014. Plant litter. Decomposition, humus formation, carbon sequestration. Third ed. Springer-Verlag Berlin Heidelberg.

    Cesco, S., T. Mimmo, G. Tonon, N. Tomasi, R. Pinton, R. Terzano, G. Neumann, L. Weisskopf, G. Renella, L. Landiand and P. Nannipieri. 2012. Plant-borne flavonoids released into the rhizosphere: impact on soil bio-activities related to plant nutrition. A review. Biol. Fertil. Soils. 48(2): 123–149.
    DOI: 10.1007/s00374-011-0653-2View Article Google Scholar

    Chae, H.M., S.H. Choi, S.H. Lee, S. Cha, K.C. Yang and J.K. Shim. 2019. Effect of litter quality on needle decomposition for four pine species in Korea. Forests 10(5): e371.
    DOI: 10.3390/f10050371View Article Google Scholar

    Chapman, S.K., G.S. Newman, S.C. Hart, J.A. Schweitzer and G.W. Koch. 2013. Leaf litter mixtures alter microbial community development: Mechanisms for non-additive effects in litter decomposition. PLoS One 8(4): e62671.
    DOI: 10.1371/journal.pone.0062671View Article Google Scholar

    Chen, F., G.G. Wang, X. Fang, S. Wan, Y. Zhang and C. Liang. 2019. Nitrogen deposition effect on forest litter decomposition is interactively regulated by endogenous litter quality and exogenous resource supply. Plant Soil 437(1-2): 413 –426.
    DOI: 10.1007/s11104-019-04006-zView Article Google Scholar

    Chen, Y., S. Ma, J. Sun, X. Wang, G. Cheng and X. Lu. 2017. Chemical diversity and incubation time affect non-additive responses of soil carbon and nitrogen cycling to litter mixtures from an alpine steppe soil. Soil Biol. Biochem. 109: 124–134.
    DOI: 10.1016/j.soilbio.2017.02.007View Article Google Scholar

    Chomel, M., M. Guittonny-Larchev?que, C. Fernandez, C. Gallet, A. DesRochers, D. Par?, B. G. Jackson and V. Baldy. 2016. Plant secondary metabolites: A key driver of litter decomposition and soil nutrient cycling. J. Ecol. 104(6): 1527–1541.
    DOI: 10.1111/1365-2745.12644View Article Google Scholar

    Fioretto, A., S. Papa, A. Pellegrino and A. Fuggi. 2007. Decomposition dynamics of Myrtus communis and Quercus ilex leaf litter: Mass loss, microbial activity and quality change. Appl. Soil Ecol. 36(1): 32–40.
    DOI: 10.1016/j.apsoil.2006.11.006View Article Google Scholar

    Garc?a-Palacios, P., E.A. Shaw, D.H. Wall and S. H?ttenschwiler. 2016. Temporal dynamics of biotic and abiotic drivers of litter decomposition. Ecol. letter 19(5): 554–563.
    DOI: 10.1111/ele.12590View Article Google Scholar

    Go??biewski, M., A. Tarasek, M. Sikora, E. Deja-Sikora, A. Tretyn and M. Nikli?ska. 2019. Rapid microbial community changes during initial stages of pine litter decomposition. Microbial Ecol. 77(1): 56–75
    DOI: 10.1007/s00248-018-1209-xView Article Google Scholar

    Gu, Y., T. Li, Q. Tao and L. Wang. 2020. Comparison on early decomposition process of forest litter from litter bag and natural environment. J. Zhejiang For. Sci. Technol. 40: 1-8.

    Hofstetter, V., B. Buyck, G. Eyssartier, S. Schnee and K. Gindro. 2019. The unbearable lightness of sequenced-based identification. Fungal Divers. 96(1): 243–284.
    DOI: 10.1007/s13225-019-00428-3View Article Google Scholar

    Huang, Y., X. Yang, D. Zhang and J. Zhang. 2020. The effects of gap size and litter species on colonization of soil fauna during litter decomposition in Pinus massoniana plantations. Appl. Soil Ecol. 155: 103611.
    DOI: 10.1016/j.apsoil.2020.103611View Article Google Scholar

    Jatav, B., T. Sharma and S. Dassani. 2020. Succession of microfungi on leaf litter of Acacia catechu in Datia, Madhya Pradesh, India. J. Pure Appl. Microbiol. 14(1): 581–590.
    DOI: 10.22207/JPAM.14.1.60View Article Google Scholar

    Jia, B. 2019. Litter decomposition and its underlying mechanisms. Chinese J. Plant Ecol. 43(8): 648–657.
    DOI: 10.17521/cjpe.2019.0097View Article Google Scholar

    Jing, H., P. Zhang, J. Li, X. Yao, G. Liu and G. Wang. 2019. Effect of nitrogen addition on the decomposition and release of compounds from fine roots with different diameters: The importance of initial substrate chemistry. Plant Soil 438(1-2): 281–296.
    DOI: 10.1007/s11104-019-04017-wView Article Google Scholar

    Jurk?ien?, G., D. Janu?auskait?, K. Armolaitis and V. Baliuckas. 2017. Leaf litterfall decomposition of pedunculate (Quercus robur l.) and sessile (Q. Petraea [matt.] liebl.) oaks and their hybrids and its impact on soil microbiota. Dendrobiology 78: 51–62.
    DOI: 10.12657/denbio.078.006View Article Google Scholar

    Klich, M.A. 2002. Biogeography of Aspergillus species in soil and litter. Mycologia 94(1): 21–27.
    DOI: 10.2307/3761842View Article Google Scholar

    Krishna, M. and M. Mohan. 2017. Litter decomposition in forest ecosystems: A review. Energy Ecol. Environ. 2(4): 236–249.
    DOI: 10.1007/s40974-017-0064-9View Article Google Scholar

    Levi, E., S. Archer, H. Throop and C. Rasmussen. 2020. Soil-litter mixing promotes decomposition and soil aggregate formation on contrasting geomorphic surfaces in a shrub-invaded sonoran desert grassland. Plant Soil 450(1-2): 397–415.
    DOI: 10.1007/s11104-020-04508-1View Article Google Scholar

    Li, S., Y. Tong and Z. Wang. 2017. Species and genetic diversity affect leaf litter decomposition in subtropical broadleaved forest in southern China. J. Plant Ecol. 10(1): 232–241.
    DOI: 10.1093/jpe/rtw122View Article Google Scholar

    Liu, Y., Z. Cui, Z. Huang, H.-T. Miao and G.-L. Wu. 2019. The influence of litter crusts on soil properties and hydrological processes in a sandy ecosystem. Hydrol. Earth Sys. Sci. 23(5): 2481–2490.
    DOI: 10.5194/hess-23-2481-2019View Article Google Scholar

    Lu, W., N. Liu, Y. Zhang, J. Zhou, Y. Guo and X. Yang. 2017. Impact of vegetation community on litter decomposition: Evidence from a reciprocal transplant study with 13C labeled plant litter. Soil Biol. Biochem. 112: 248–257.
    DOI: 10.1016/j.soilbio.2017.05.014View Article Google Scholar

    Lv, W., L. Zhang, H. Niu, B. Li, Q. Wang, Y. Zhou, Y. Wang, L. Jiang, P. Liu, H. Hong, S. Jia, C. Luo, D. Tsechoe, H. Zhou and H. Hong. 2020. Non-linear temperature sensitivity of litter component decomposition under warming gradient with precipitation addition on the Tibetan Plateau. Plant Soil 448(1-2): 335–351.
    DOI: 10.1007/s11104-020-04431-5View Article Google Scholar

    Moore, T.R., J. Trofymow, C. Prescott, B. Titus and C. W. Group. 2017. Can short-term litter-bag measurements predict long-term decomposition in northern forests? Plant Soil 416(1-2): 419–426.
    DOI: 10.1007/s11104-017-3228-7View Article Google Scholar

    Osono, T., C. To-Anun, Y. Hagiwara and D. Hirose. 2011. Decomposition of wood, petiole and leaf litter by Xylaria species from northern Thailand. Fungal. Ecol. 4(3): 210–218.
    DOI: 10.1016/j.funeco.2010.11.003View Article Google Scholar

    Osono, T., D. Hirose and R. Fujimaki. 2006. Fungal colonization as affected by litter depth and decomposition stage of needle litter. Soil Biol. Biochem. 38(9): 2743–2752.
    DOI: 10.1016/j.soilbio.2006.04.028View Article Google Scholar

    Otaki, M. and S. Tsuyuzaki. 2019. Succession of litter-decomposing microbial organisms in deciduous birch and oak forests, northern Japan. Acta Oecol. 101: 103485.
    DOI: 10.1016/j.actao.2019.103485View Article Google Scholar

    Panteleit, J., F.G. Horgan, M. T?rke, A. Schmidt, M. Sch?dler, M. Bacht, R. Brandl and S. Hotes. 2018. Effects of detritivorous invertebrates on the decomposition of rice straw: evidence from a microcosm experiment. Paddy Water Environ. 16(2): 279–286
    DOI: 10.1007/s10333-017-0625-8View Article Google Scholar

    Pierist?, M., M. Chauvat, T.K. Kotilainen, A.G. Jones, M. Aubert, T.M. Robson and E. Forey. 2019. Solar UV-A radiation and blue light enhance tree leaf litter decomposition in a temperate forest. Oecologia 191(1): 191–203.
    DOI: 10.1007/s00442-019-04478-xView Article Google Scholar

    Prescott, C.E. and S.J. Grayston. 2013. Tree species influence on microbial communities in litter and soil: Current knowledge and research needs. Forest Ecol. Manag. 309: 19–27.
    DOI: 10.1016/j.foreco.2013.02.034View Article Google Scholar

    Purahong, W., T. Wubet, G. Lentendu, M. Schloter, M.J. Pecyna, D. Kapturska, M. Hofrichter, D. Kruger and F. Buscot. 2016. Life in leaf litter: Novel insights into community dynamics of bacteria and fungi during litter decomposition. Molecular Ecol. 25(16): 4059–4074.
    DOI: 10.1111/mec.13739View Article Google Scholar

    Sauvadet, M., N. Fanin, M. Chauvat and I. Bertrand. 2019. Can the comparison of above-and below-ground litter decomposition improve our understanding of bacterial and fungal successions? Soil Biol. Biochem. 132: 24–27.
    DOI: 10.1016/j.soilbio.2019.01.022View Article Google Scholar

    Smith, S.W., S.J. Woodin, R.J. Pakeman, D. Johnson and R. Van Der Wal. 2014. Root traits predict decomposition across a landscape?scale grazing experiment. New Phytol. 203(3): 851–862.
    DOI: 10.1111/nph.12845View Article Google Scholar

    Song, F., X. Tian, X. Fan and X. He. 2010. Decomposing ability of filamentous fungi on litter is involved in a subtropical mixed forest. Mycologia. 102(1): 20–26.
    DOI: 10.3852/09-047View Article Google Scholar

    Song, F., X. Tian, Z. Li, C. Yang, B. Chen, J. Hao and J. Zhu. 2004. Diversity of filamentous fungi in organic layers of two forests in Zijin Mountain. J. For. Res. 15(4): 273–279.
    DOI: 10.1007/BF02844951View Article Google Scholar

    Tian, K., X. Kong, J. Gao, Y. Jia, H. Lin, Z. He, Y. Ji, Z. Bei and X. Tian. 2018. Local root status: A neglected bio-factor that regulates the home-field advantage of leaf litter decomposition. Plant Soil 431(1-2): 175–189.
    DOI: 10.1007/s11104-018-3757-8View Article Google Scholar

    Tu, L., H. Hu, T. Hu, J. Zhang, L. Liu, R. Li, H. Dai and S. Luo. 2011. Decomposition of different litter fractions in a subtropical bamboo ecosystem as affected by experimental nitrogen deposition. Pedosphere 21(6): 685–695.
    DOI: 10.1016/S1002-0160(11)60171-9View Article Google Scholar

    Wang, W., Q. Zhang, X. Sun, D. Chen, H. Insam, R.T. Koide and S. Zhang. 2020. Effects of mixed-species litter on bacterial and fungal lignocellulose degradation functions during litter decomposition. Soil Biol. Biochem. 141: e107690.
    DOI: 10.1016/j.soilbio.2019.107690View Article Google Scholar

    Yan, H., X. Gu and H. Shen. 2010. Microbial decomposition of forest litter: A review. Chinese J. Ecol. 29: 1827–1835.

    Zhang, X., B. Wang and Z. Liu. 2019. Impacts of plant secondary metabolites from conifer litter on the decomposition of Populus purdomii litter. J. For. Res. 30(6): 2237–2245.
    DOI: 10.1007/s11676-018-0766-7View Article Google Scholar

    Zhang, X., Z. Liu, Q. Yu, N.T. Luc, Y. Bing, B. Zhu and W. Wang. 2015. Effect of petroleum on decomposition of shrub-grass litters in soil in northern Shaanxi of China. J. Environ. Sci. 33: 245–253.
    DOI: 10.1016/j.jes.2014.12.013View Article Google Scholar

    Zhao, C., J. Long, H. Liao, C. Zheng, J. Li, L. Liu and M. Zhang. 2019. Dynamics of soil microbial communities following vegetation succession in a karst mountain ecosystem, southwest China. Sci. Rep. 9(1): e2160.
    DOI: 10.1038/s41598-018-36886-zView Article Google Scholar

    Zheng, J. and S. Han. 2016. Nitrogen transfer in the litter-soil interface ontinuum of the temperate forest. J. Beijing Forestry Univ. 38: 116–122.

    Zhong, Y., W. Yan, R. Wang, W. Wang and Z. Shangguan. 2018. Decreased occurrence of carbon cycle functions in microbial communities along with long-term secondary succession. Soil Biol. Biochem. 123: 207–217.
    DOI: 10.1016/j.soilbio.2018.05.017View Article Google Scholar

    Zuo, L., R. Huang, Q. Zhang, P. Chen and L. Li. 2020. The diversity of endophytic and epiphytic fungi of the litter in differently aged Casuarina equisetifolia forests in Hainan island. Acta Ecol. Sin. 40(17): 6086–6095.
    DOI: 10.5846/stxb201906121244View Article Google Scholar