Research Paper

Primulina rufipes, a new species of Gesneriaceae from Guangxi, China

Yu-Lan Su, Zhang-Ping Huang, Ping Yang, Wei-Bin Xu, Yan Liu

Published on: 07 January 2022

Page: 40 - 46

DOI: 10.6165/tai.2022.67.40


Primulina rufipes Y.L. Su, P. Yang & Yan Liu (Gesneriaceae), a new species from Guangxi, China, is described and illustrated. Molecular phylogenetic analyses unambiguously put this species in Primulina. Morphological observations and comparisons showed that P. rufipes is similar to P. huaijiensis Z.L. Ning & J. Wang, differing by all over the plant densely pubescent and glandular-puberulent, leaf blade elliptic to rhomboid-ovate, and obliquely campanulate corolla without appendages. P. rufipes is also similar to P. yulinensis Ying Qin & Yan Liu and P. gongchengensis Y.S. Huang & Yan Liu in vegetative characters, but its obliquely campanulate corolla is obviously different from the latter two species.

Keyword: Karst cave, limestone flora, morphology, molecular phylogeny, Primulina gongchengensis, P. huijiensis, P. yulinensis

Literature Cited

Doyle, J.-J. and J.-L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.

Edgar, R.-C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32(5): 1792–1797.
DOI: 10.1093/nar/gkh340View Article Google Scholar

Fang, D. and D.-H. Qin. 2004. Wentsaiboea D. Fang et D.H. Qin, a new genes of Gesneriaceae from Guangxi, China. Acta Phytotax. Sin. 42: 533–536.

Guo, J., B. Pan, J. Liu, W.-B. Xu and K.-F. Chung. 2015. Three new species of Primulina (Gesneriaceae) from limestone karsts of China based on morphological and molecular evidence. Bot. Stud. 56(1): 34–46.
DOI: 10.1186/s40529-015-0115-5View Article Google Scholar

Hall, T.-A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41: 95–98.

Hance, H.-F. 1883. New Chinese Cyrtandreae. J. Bot. 21: 165–170. IPNI. 2020. The International Plant Names Index. [accessed 20 December 2020]

Liu, Yan, W.-B. Xu and B. Pan. 2010. Wentsaiboea tiandengensis sp. nov. and W. luochengensis sp. nov. (Gesneriaceae) from Karst caves in Guangxi, southern China. Nordic J. Bot. 28(6): 739–745.
DOI: 10.1111/j.1756-1051.2010.00893.xView Article Google Scholar

M?ller, M., M. Pfosser, C.-G. Jang, V. Mayer, A. Clark, M.-L. Hollingsworth, M.-H.-J. Barfuss, Y.-Z. Wang, M. Kiehn and A. Weber. 2009. A preliminary phylogeny of the Didymocarpoid Gesneriaceae based on three molecular data sets: incon gruence with available tribal classifications. Amer. J. Bot. 96(5): 989–1010.
DOI: 10.3732/ajb.0800291View Article Google Scholar

Ning, Z.-L., G.-F. Li, J. Wang, J.-F. Smith, H. Rasolonjatovo and M. Kang. 2013. Primulina huaijiensis (Gesneriaceae), a new species from Guangdong, China. Ann. Bot. Fenn. 50(1-2): 119–122.
DOI: 10.5735/085.050.0124View Article Google Scholar

Posada, D. and K.-A. Crandall. 1998. ModelTest: testing the model of DNA substitution. Bioinformatics 14(9): 817–818.
DOI: 10.1093/bioinformatics/14.9.817View Article Google Scholar

Ronquist, F., M. Teslenko, P.-V.-D. Mark, D.-L. Ayres, A. Darling, S. H?hna, B. Larget, L. Liu, M.-A. Suchard and J.-P. Huelsenbeck. 2012. MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice. Syst. Biol. 61(3): 539–542.
DOI: 10.1093/sysbio/sys029View Article Google Scholar

Smissen, R.-D., I. Breitwieser and J.-M. Ward. 2004. Phylogenetic implications of trans-specific chloroplast DNA sequence polymorphism in New Zealand Gnaphalieae (Asteraceae). Plant Syst. Evol. 249(1-2): 37–53.
DOI: 10.1007/s00606-004-0209-0View Article Google Scholar

Stamatakis, A. 2006. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22(21): 2688–2690.
DOI: 10.1093/bioinformatics/btl446View Article Google Scholar

Wang, W.-T. 1986. Notulae de Gesneriaceis Sinensibus (VII). Guihaia 6: 1–15

Wang, Y.-Z., R.-B. Mao, Y. Liu, J.-M. Li, Y. Dong, Z.-Y. Li, and J.-F. Smith. 2011. Phylogenetic reconstruction of Chirita and allies (Gesneriaceae) with taxonomic treatments. J. Syst. Evol. 49(1): 50–64.
DOI: 10.1111/j.1759-6831.2010.00113.xView Article Google Scholar

Weber, A., D.-J. Middleton, A. Forrest, R. Kiew, C.-L. Lim, A.-R. Rafdah, S. Sontag, P. Triboun, Y.-G. Wei, T.-L. Yao and M. M?ller. 2011. Molecular systematics and remodeling of Chirita and associated genera (Gesneriaceae). Taxon 60(3): 767–790.
DOI: 10.1002/tax.603012View Article Google Scholar

Xu, W.-B., B. Pan, Y. Liu, C.-I. Peng and K.-F. Chung. 2012 Two new species, Primulina multifida and P. pseudomollifolia (Gesneriaceae), from karst caves in Guangxi, China. Bot. Stud. 53: 165–175.

Xu, W.-B., H. Chang, J. Huang and K.-F. Chung. 2019. Molecular systematics of Chiritopsis-like Primulina (Gesneriaceae): one new species, one new name, two new combinations, and new synonyms. Bot. Stud. 60(1): 13–21.
DOI: 10.1186/s40529-019-0266-xView Article Google Scholar

Wen, F., Y.-G. Wei, L.-F. Fu, Z.-B. Xin, S. Li, Z.-J. Huang, Y.-Z. Ge, D.-C. Meng. 2020. The Checklist of Gesneriaceae in China. [accessed 20 December 2020]

Wu, H.-T., S.-B. Zhou, W. Ma, O.-W. Wang. 2017. Primulina dichroantha (Gesneriaceae), a New Species from a Karst Cave from Guangxi, China. Ann. Bot. Fenn. 54(1-3): 95–98.
DOI: 10.5735/085.054.0314View Article Google Scholar