Prunus sunhangii was recently described as an independent species related to P. cerasoides with molecular evidence from ITS and four cpDNA sequences (psbA-trnH, rpS16, trnL-trnF and trnS-trnG). However, while repeating the phylogenetic analysis of P. sunhangii, we found some flaws in the previous analysis, including the extremely long branch of the outgroup and the poor sequence quality, which may affect the reliability of the result. Therefore, a new phylogenetic analysis was performed to verify the identity of P. sunhangii with a new data matrix and morphological comparisons. The results showed that P. sunhangii forms a monophyletic clade with P. itosakura var. ascendens with high support and P. sunhangii is similar with P. itosakura var. ascendens in every morphological aspect. We concluded that P. sunhangii is conspecific with P. itosakura var. ascendens and should be synonymized with the latter species.
Kato, S., A. Matsumoto, K. Yoshimura, T. Katsuki, K. Iwamoto, T. Kawahara, Y. Mukai, Y. Tsuda, S. Ishio, K, Nakamura, K. Moriwaki, T. Shiroishi, T. Gojobori and H. Yoshimaru. 2014. Origins of Japanese flowering cherry (Prunus subgenus Cerasus) cultivars revealed using nuclear SSR markers. Tree Genet. Genomes 10(3): 477–487. DOI: 10.1007/s11295-014-0697-1View ArticleGoogle Scholar
Katoh, K., K. Misawa, K.-I. Kuma and T. Miyata. 2002. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 30(14): 3059–3066. DOI: 10.1093/nar/gkf436View ArticleGoogle Scholar
Katsuki, T. 2009. Flowering cherries in Japan. Gakken, Tokyo. 264pp. (In Japanese)
Kuitert, W. 1999. Japanese flowering cherries. Timber Press, Portland, Oregon, USA. 395pp.
Lanfear, R., P. B. Frandsen, A. M. Wright, T. Senfeld, and B. Calcott. 2017. PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Mol. Biol. Evol. 34(3): 772–773. DOI: 10.1093/molbev/msw260View ArticleGoogle Scholar
Li, C.-L. and B. Bartholomew. 2003. Cerasus. In: Wu, Z.-Y. and P. H. Raven eds., Flora of China, 9: 404–420. Science Press & Missouri Botanical Garden Press, Beijing, China and St. Louis, USA.
Makino, T. 1940. An Illustrated Flora of Nippon, with the Cultivated and Naturalized Plants. Hokuryukan, Tokyo, Japan. 1218pp.
Ohwi, J. 1965. Prunus. In: Meyer F. G. and E. H. Walker eds., Flora of Japan by Jisaburo Ohwi (in English), 541–545. Smithsonian Institution, Washington, USA.
Ronquist, F. and J. P. Huelsenbeck. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19(12): 1572–1574. DOI: 10.1093/bioinformatics/btg180View ArticleGoogle Scholar
Ronquist, F., M. Teslenko, P. Van Der Mark, D. L. Ayres, A. Darling, S. H?hna, B. Larget, L. Liu, M. A. Suchard and J. P. Huelsenbeck. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61(3): 539–542. DOI: 10.1093/sysbio/sys029View ArticleGoogle Scholar
Talavera, G. and J. Castresana. 2007. Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst. Biol. 56(4): 564–577. DOI: 10.1080/10635150701472164View ArticleGoogle Scholar
Wang, X.-R. 2000. Studies on classification of the Group of Cerasus subhirtella (Miq.) Sok. and its Usage. J. Nanjing Forest. Univ. (Natural Science Edition) 24: 44–46.
Yu, T.-T. and C.-L. Li. 1986. Cerasus. In: Yu, T.-T. ed., Flora Reipublicae Popularis Sinicae. 38: 41–89. Science Press, Beijing.
Zhang, X.S., Z.-L. Jiang, Z. Yusupov, M.-H. Zhang, D.-G. Zhang, K. Tojibaev, Y. Meng and T. Deng. 2019. Prunus sunhangii: A new species of Prunus from central China. Plant Divers. 41(1): 19–25. DOI: 10.1016/j.pld.2019.01.003View ArticleGoogle Scholar
Zhang, D., F.-L. Gao, I. Jakovli?, H. Zou, J. Zhang, W.-X. Li, and G.-T. Wang. 2020. PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Mol. Ecol. Resour. 20(1): 348–355. DOI: 10.1111/1755-0998.13096View ArticleGoogle Scholar
Copyright @ Taiwania Editorial Office. All rights reserved.
Published by College of Life Science, National Taiwan University & Biodiversity Association of Taiwan, Taiwan
Contact address: Institute of Ecology and Evolutionary Biology, College of Life Science, National Taiwan University, No.1, Sec.4, Roosevelt Road, Taipei 10617, Taiwan