Abstract

Acid phosphatase in different tissue of rice (Oryza sativa) was studied with polyacrylamide gel electrophoresis and enzyme assay. The zymogram of acid phosphatase showed different isozyme patterns in roots, leaves, seedlings and calli. One of the medium mobility isozymes, cell-wall-containing enzyme, commonly occurred in all tissues. Some isozyme had tissue-specificity. The optimal pH of acid phosphatase was around 5. Enzyme activity was strongly inhibited by HgCl2, (NH4)4Mo7O24, NaF, CuSO4 and KH2PO4. In germinating seeds, the increasing activity of acid phosphatase related with transportation of phosphates and store food had been suggested.

中文摘要

利用電泳分析法及酵素檢定法探討水道不同組織中的酸性磷解酶。同功酸性磷解酶的類型在根、葉，幼苗及癒合組織都不相同。有些同功酶具組織特異性，一中度移速的同功酶在所有組織裏的細胞壁都有存在。酸性磷解酶最適酸鹼度是在5左右。HgCl2, (NH4)4Mo7O24, NaF, CuSO4 及 KH2PO4都具有很強地抑住酵素活性作用。在發芽種子中酸性磷解酶被推測與儲藏物質和磷酸鹽的輸送有關。