Research Paper

A new natural hybrid Goodyera ×kazumae (Orchidaceae) from Tomakomai City, Hokkaido Prefecture, Japan

Kenji Suetsugu, Shun K. Hirota, Yoshihisa Suyama

Published on: 26 July 2024

Page: 309 - 316

DOI: 10.6165/tai.2024.69.309

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    Abstract

    Several individuals of an unidentified Goodyera taxon were discovered in Tomakomai City, Hokkaido Prefecture, Japan. Detailed morphological analysis suggests that this taxon is a natural hybrid between Goodyera schlechtendaliana and Goodyera henryi, based on characteristics such as leaf margins, texture, venation patterns, rachis length, hair morphology on the ovary, and the shapes of the lip apex and rostellum. Supporting this, molecular data from genome-wide markers using next-generation sequencing (MIG-seq data) indicate that G. ×kazumae is a hybrid between G. schlechtendaliana and G. henryi. Neighbor-Net phylogenetic analysis shows G. ×kazumae occupies an intermediate position between the putative parent species. STRUCTURE analysis further confirms that G. ×kazumae comprises genetic components from both species. Therefore, we conclude that this taxon is a natural hybrid based on comprehensive morphological and molecular evidence.

    Keyword: DNA barcoding, Goodyera henryi, Goodyera schlechtendaliana, hybridization, MIG-seq

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