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Development and characterization of EST-SSR markers in creeping mazus (Mazus miquelii), and cross-amplification in five related species

Masaya Yamamoto, Daiki Takahashi, Chih-Chieh Yu, Hiroaki Setoguchi

Published on: 18 April 2020

DOI: 10.6165/tai.2020.65.249

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2020 vol.65 no.2 pp.249-252

Abstract

Simple sequence repeat (SSR) markers were developed from expressed sequence tags (ESTs) for Mazus miquelii (Mazaceae), one of the most widespread species of the genus found in mainland China to Japan and North America, with the goal of elucidating the hidden genetic diversity and pollination ecology of the species. Of the initial 48 EST-SSR markers designed based on transcriptome data, 36 loci were successfully amplified, 16 of which were polymorphic. Polymorphisms for these markers were tested on 72 individuals from three populations in Japan. Two to eleven alleles per locus were detected, and the levels of observed and expected heterozygosity ranged from 0.181 to 0.708 and 0.154 to 0.715, respectively. Most loci were amplified successfully in five related Japanese and Taiwanese species. These markers will empower ecological and evolutionary studies in the creeping mazus and facilitate the disentanglement of phylogenetic relationships with related species.

Keyword: microsatellite, genetic diversity, expressed sequence tag, Mazus, Mazaceae

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