Research Paper

ANALYSIS ON PIGMENTS IN THE EXOCARP OF ORANGE FRUIT

Jen-Chieh Hsu, Yih-Kuang Lu and Chi-Ming Yang

Published on: March 1995

Page: 83 - 90

DOI: 10.6165/tai.1995.40.83

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1995 vol.40 no.1 pp.83-90

Abstract

We examined the chlorophyll (Chl) and carotenoid contents, and the three intermediates (protoporphyrin IX (PPIX), magnesium protoporphyrin IX (MGPP) and protochlorophyllide (Pchlide)) of Chl biosynthetic pathway in the exocarp of orange fruit during its late ripening stage. While Chl content declines by 12 folds, carotenoid content always remains similar level. meanwhile, the ratios of Chl a/b, carotenoid/Chl and Pchlide/Chl alter from 1.51 to 4.42 from 0.22 to 2.74 and from 1.00 to 0.07, respectively. It is, therefore, concluded: (a) the deficiency of carotenoid is not directly accompanied by the deficiency of Chl; (b) the degradation rate of Chl b is faster than that of Chl a; (c) the degradation rate of PPIX is slower than that of MGPP and Pchlide; and (d) as ripening takes place, the residual Pchlide is more easily forwarded to Chl.

中文摘要

本研究檢測橘皮後熟階段葉綠素和類胡蘿蔔素,及三種葉綠素生合成途徑的中間代謝物─ protoporphyrin IX (PPIX),magnesium protoporphyrin IX (MGPP) 和 protochlorophyllid (Pchlide),的含量變化。當葉綠素含量下降 12 倍,類胡蘿蔔素都保持相似的含量。同期間,葉綠素 a/b 比,類胡蘿蔔素 / 葉綠素比和 Pchlide / 葉綠素比分別自 2.51,2.22 和 1.00 改變為 4.42,2.74 和 0.07。因此,類胡蘿蔔素的缺失並不直接伴隨著葉綠素的缺失;葉綠素 b 的崩解比葉綠素 a 快速;PPIX 的崩解比 MGPP 和 Pchlide 緩慢;在後熟的最後階段,殘餘的 Pchlide 似更容易轉化為葉綠素而非崩解。

Keyword: Chlorophyll, Carotenoid, Magnesium protoporphyrin, Pigment, Proto-chloro-phyllide, Protoporphyrin IX. 葉綠素,類胡蘿蔔素,原吡啉鎂,色素,葉綠原素,原吡啉 IX。