Research Paper

Pollination ecology of Impatiens uliginosa (Balsaminaceae) endemic to China

Bai-Zhu Li, Zhao-Feng Li, Meng-Da Xiang, De-Mei Hu, Yu Li, Yin Yi, Xiao-Xin Tang

Published on: 06 July 2021

Page: 298 - 306

DOI: 10.6165/tai.2021.66.298

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    Abstract

    China is one of the five major distribution regions of Impatiens in the world, with more than 270 species distributed all over the country, especially in limestone areas such as Yunnan, Guizhou and Guangxi. In order to explore pollination ecology of Impatiens uliginosa, we conducted the following experiments: observing the floral traits and floral phenology, measuring the nectar volume and sugar concentration, counting pollen viability, deposition and removal, carrying out pollination treatments and recording floral visitors and their behavior. Results show that the average life span of a single flower is 3.23±0.68 d. The male and female phase lasted for 1.70±0.06 d and 1.53±0.63 d. The amount of nectar per flower is 6.97±0.47 ul and sugar concentration of nectar is 36.58±0.82%, respectively. Stigma is receptive on the first day of anthesis. Pollen viability is strongest on the second day of anthesis. The pollen-ovule ratio (P/O) is 3974.28±105.07. We investigated the contributions of different floral visitors. Bombus sp., Apis dorsata, Lobocla proxima, Ant sp. and Ephydra sp. are diurnal visitors. However, Bombus sp. is the most effective pollinator of I. uliginosa. Pollination treatments indicated that I. uliginosa is self-compatible but completely relies on pollinators for pollination. There is no restriction on pollination in the field and I. uliginosa can avoid inbreeding depression. This study is of great significance to enrich the biological data of pollination and protect the biodiversity of Impatiens.

    Keyword: Breeding system, Impatiens uliginosa, pollination biology, pollination treatments

    Literature Cited

    Arizmendi, M.D.C. 2001. Multiple ecological interactions: nectar robbers and hummingbirds in a highland forest in Mexico. Can. J. Zool. 79(6): 997–1006.
    DOI: 10.1139/z01-066View Article Google Scholar

    Abrahamczyk, S., A.M. Humphreys, F. Trabert, F. Droppelmann and E. Fischer. 2021. Evolution of brood-site mimicry in Madagascan Impatiens (Balsaminaceae). Perspect Plant Ecol. 49: 125590.
    DOI: 10.1016/j.ppees.2021.125590View Article Google Scholar

    Barrett, S.C.H. 2002. The evolution of plant sexual diversity. Nat. Rev. Genet. 3(4): 274–84.
    DOI: 10.1038/nrg776View Article Google Scholar

    Bradshaw, H.D. and D.W. Schemske. 2003. Allele substitution at a flower colour locus produces a pollinator shift in monkeyflowers. Nature 426(6963): 176–178.
    DOI: 10.1038/nature02106View Article Google Scholar

    Chen, Y.-L. 2001. Balsaminaceae. In: Flora Reipublicae Popularis Science, Science Press, Beijing. pp. 1–397.

    Charlesworth, D. and B. Charlesworth. 1987. Inbreeding depression and its evolutionary consequences. Annu. Rev. Ecol Syst. 18(1): 237–268.
    DOI: 10.1146/annurev.es.18.110187.001321View Article Google Scholar

    Christenhusz, M. and J.W. Byng. 2016. The number of known plants species in the world and its annual increase. Phytotaxa 261(3): 201–217.
    DOI: 10.11646/phytotaxa.261.3.1View Article Google Scholar

    Dafni, A. 1992. Pollination Ecology-A Practical Approach: Oxford University Press.

    Faegri, K. and L.V.D. Pijl. 1966. The Principles of Pollination Ecology. Oxford: Pergamon Press.

    Greywilson, C. 1980. Impatiens of Africa. Rotterdam: Belkema Press.

    Huang, S.-Q. and Y.-H. Guo. 2000. New advances in pollination biology and studies in China. Chinese Sci Bull. 45(16): 1441–1447.
    DOI: 10.1007/BF02898884View Article Google Scholar

    Hodges, C.M. 1985. Bumble Bee Foraging: The Threshold Departure Rule. Ecology 66(1): 179–187.
    DOI: 10.2307/1941318View Article Google Scholar

    Huang, S.-Q. 2007. Studies on plant-pollinator interaction and its significances. Biodiversity Science 15(6): 569–575.
    DOI: 10.1360/biodiv.070294View Article Google Scholar

    Huang, S.-Q. 2014. Most effective pollinator principle of floral evolution: evidence and query. Chinese Bull Life Sciences 26(2): 118–124.

    Janssens, S. B., S.-W. Yi, Y.-M. Yuan, A. Nagels, E.F. Smets and S. Huysmans. 2012. A total evidence approach using palynological characters to infer the complex evolutionary history of the Asian Impatiens (Balsaminaceae). Taxon 61(2): 355–367.
    DOI: 10.1002/tax.612007View Article Google Scholar

    Johnson, S.D. and F.P. Schiestl. 2016. Floral Mimicry. Oxford University Press.

    Kato, M. 1988. Bumblebee visits to Impatiens spp.:pattern and efficiency. Oecologia 76(3): 364–370.
    DOI: 10.1007/BF00377030View Article Google Scholar

    Kato, M., T. Itino, M. Hotta and T. Inoue. 1991. Pollination of four Sumatran Impatiens species by hawkmoths and bees. Tropics 1(1): 59–73.
    DOI: 10.3759/tropics.1.59View Article Google Scholar

    Klinkhamer, P.G.L. and T.J. de. Jong. 1993. Attractiveness to pollinators: a plant's dilemma. Oikos 66(1): 180–184.
    DOI: 10.2307/3545212View Article Google Scholar

    Li, Y.-H. and Q. Wang. 2014. Pollen Limitation of the Seed Setting of Impatiens oxyanthera (Balsaminaceae). J. Sichuan For. Sci. Technol. 35(4): 17–22.

    Liu, C.-Q. and S.-Q. Huang. 2013. Floral divergence, pollinator partitioning and the spatiotemporal pattern of plant-pollinator interactions in three sympatric Adenophora species. Oecologia 173(4): 1411–1423.
    DOI: 10.1007/s00442-013-2723-7View Article Google Scholar

    Melo, M., P. Taucce and E. L. Borba. 2011. Reproductive biology and isolation mechanisms in rupicolous species of the Acianthera prolifera complex (Orchidaceae) occurring in southeastern Brazi. Plant Syst. Evol. 293(1-4): 161–176.
    DOI: 10.1007/s00606-011-0437-zView Article Google Scholar

    Mao, Z.-B., B. Cedric and X.-J. Ge. 2011. Pollination ecology and breeding system of Impatiens lateristachys (Balsaminaceae) endemic to China. Guihaia. 31: 160–166.

    Miller, T.E. and J. Travis. 1996. The evolutionary role of indirect effects in communities. Ecology 77(5): 1329–1335.
    DOI: 10.2307/2265530View Article Google Scholar

    Maloof, J.E. 2001. The effects of a bumble bee nectar robber on plant reproductive success and pollinator behavior. Am. J. Bot. 88(11): 1960–1965.
    DOI: 10.2307/3558423View Article Google Scholar

    Olesen, J.M. 1996. Supplement: Special Publication Number 2: Proceedings of the Eickwort Memorial Symposium From Navet? to Experience: Bumblebee Queens (Bombus terrestris) Foraging on Corydalis cava (Fumariaceae). J Kansas Entomol Soc. 69(4): 274–286.

    Pyke, G. H. 1982. Foraging in bumblebees: rule of departure from an inflorescence. Can. J. Zool. 60(3): 417–428.
    DOI: 10.1139/z82-057View Article Google Scholar

    Robertson, C. 1928. Flowers and insects. Ecology 9(4): 505–526.
    DOI: 10.2307/1929418View Article Google Scholar

    Randall, L. J. and K.W. Hilu. 1990. Interference through improper pollen transfer in mixed stands of Impatiens capensis and I. pallida (Balsaminaceae). Am. J. Bot. 77(7): 939–944.
    DOI: 10.2307/2444509View Article Google Scholar

    Reynolds, R. J., A. A. R. Kula, C. B. Fenster and M. R. Dudash. 2012. Variable nursery pollinator importance and its effect on plant reproductive success. Oecologia 168(2): 439–448.
    DOI: 10.1007/s00442-011-2095-9View Article Google Scholar

    Ruchisansakun, S., P. Suksathan, P. Triboun and C. Sinrotthanakorn. 2021. Impatiens capillipes (Balsaminaceae), a new record for Thailand. Thai Forest Bulletin (Botany) 48: 48–51.
    DOI: 10.20531/tfb.2020.48.1.08View Article Google Scholar

    Rust, R. W. 1977. Pollination in Impatiens capensis and Impatiens pallida (Balsaminaceae). Bull. Torrey Bot. Club. 104(4): 361–367.
    DOI: 10.2307/2484781View Article Google Scholar

    Schemske, D. W. 1978. Evolution of reproductive characteristics in Impatiens (Balsaminaceae): the significance of cleistogamy and chasmogam. Ecology 59(3): 596–613.
    DOI: 10.2307/1936588View Article Google Scholar

    Schmitt, J. and D. W. Ehrhardt. 1990. Enhancement of inbreeding depression by dominance and suppression in Impatiens capensis. Evolution 44(2): 269–278.
    DOI: 10.2307/2409406View Article Google Scholar

    Sato, H. 2002. The role of autonomous self-pollination in floral longevity in varieties of Impatiens hypophyla (Balsaminaceae). Am J Bot. 89(2): 263–269.
    DOI: 10.3732/ajb.89.2.263View Article Google Scholar

    Twasuda, M. and T, Yahara. 1994. Reproductive ecology of a cleistogamous annual, Impatiens noli-tangere L. occuring under different environmental conditions. Ecol Res. 9(1): 67–75.
    DOI: 10.1007/BF02347243View Article Google Scholar

    Tian, J.-P., K.-M. Liu and G.-W. Hu. 2004. Pollination ecology and pollination system of Impatiens reptans (Balsaminaceae) endemic to China. Ann Bot-London. 93(2): 167–175.
    DOI: 10.1093/aob/mch013View Article Google Scholar

    Tang, Y.-F., Y. Fang, C.-Q. Liu, Q.-B. Lu and X.-H. Hu. 2020. The long spur of Impatiens macrovexilla may reflect adaptation to diurnal hawkmoth pollinators despite diversity of floral visitors. Flora 266: 151599.
    DOI: 10.1016/j.flora.2020.151599View Article Google Scholar

    Tong, Z.-Y., H.-L. Xu and S.-Q. Huang. 2018. Examining methodologies of pollinator detection in the field. Biodiversity Science 26(5): 9–20.
    DOI: 10.17520/biods.2017334View Article Google Scholar

    Tuno, N., M. Nitta, M. Kobayashi and K. Kitabayashi. 2019. Diversity and host associations of dipteran insects exploiting fungal fruiting bodies in Hokuriku, central Japan. Entomol. Sci. 22(2): 161–166.
    DOI: 10.1111/ens.12349View Article Google Scholar

    Wilson, P. and J.D. Thomson. 1991. Heterogeneity among floral visitors leads to discordance between removal and deposition of pollen. Ecology 72(4): 1503–1507.
    DOI: 10.2307/1941124View Article Google Scholar

    Wilson, P. 1995. Selection for pollination success and the mechanical fit of Impatiens flowers around bumblebee bodies. Biol. J. Linn Soc. 55(4):355–383.
    DOI: 10.1006/bijl.1995.0047View Article Google Scholar

    Walters, B.B. and E.W. Stiles. 1966. Effective of canopy gaps and flowers patch size on pollinator visitation of Impatiens capensis. Bull. Torrey Bot. Club. 123(3): 184–188.
    DOI: 10.2307/2996793View Article Google Scholar

    Waller, D.M. 1980. Environmental determinants of outcrossing in Impatiens capensis (Balsaminaceae). Evolution 34(4): 747–761.
    DOI: 10.2307/2408029View Article Google Scholar

    Willmer, P. 2011. Pollination and floral ecology. Princeton University Press.

    Webb, C. J. and D. G. Lloyd. 1986. The avoidance of interference between the presentation of pollen and stigmas in angiosperms ii. herkogamy. New Zeal J Bot. 24(1): 163–178.
    DOI: 10.1080/0028825X.1986.10409726View Article Google Scholar

    Wang, X.-Y., X.-X. Zhu, J. Yang, Y.-J. Liu and X.-X. Tang. 2019. Variation in style length and the effect on reproduction success in Chinese plum (Armeniaca mume). Biodiversity Science. 27(2): 159–167.
    DOI: 10.17520/biods.2018317View Article Google Scholar

    Xiao, L.-X. and K.-M. Liu. 2009. Floral traits and pollination system of Impatiens chinensis (Balsaminaceae). Bullentin of Botanical Research 29(2): 164–168.

    Xiao, L.-X. 2009. The pollination biology of four species of Impatiens L. Hunan Normal University.

    Yu, S.-X. 2012. Balsaminaceae of China. Beijing:Peking University Press.

    Yu, S.-X., S. B. Janssens, X.-Y. Zhu, M. Lid?n, T.-G. Gao and W. Wang. 2016. Phylogeny of Impatiens (Balsaminaceae): integrating molecular and morphological evidence into a new classification. Cladistics 32(2): 179–197.
    DOI: 10.1111/cla.12119View Article Google Scholar

    Zimmerman, M. and S. Cook. 1985. Pollinator foraging, experimental nectar-robbing and plant fitness in Impatiens capensis. Am Midl Nat. 113(1): 84–91.
    DOI: 10.2307/2425350View Article Google Scholar

    Zhong, Y.-F., Z. Zhang, X.-Q. Song and Z.-D. Zhou. 2014. Pollination biology of Impatiens hainanensis (Balsaminaceae) populations at different altitudes. Biodiversity Science 22(4): 467–475.
    DOI: 10.3724/SP.J.1003.2014.13243View Article Google Scholar

    Zhang, W.-W. and Y.-S. Li. 2011. Chinese Insects Illustrated. Chongqing: Chongqing University Press.

    Zhang, Y.-W., Y. Wang and Y.-H. Guo. 2006. Effects of nectar-robbing on plant reproduction and ecology. J Plant Ecol. 30(4): 443–449.

    Zhang, T., X.-X. Tang and Q. Fang. 2021. Pollinator sharing among co-flowering plants mediates patterns of pollen transfer. Alpine Bot. 131: .