Abstract

Leafy gametophytes of Haplomitrium rotundifolium developed from spores have been completed in antiseptic culture in the present laboratory (6), (7) & (8). However, no sexuality occurred in these artificially developed gametophytes. This study attempts to detect whether external factors such as light sources and chemicals may bring some morphological or physiological changes, which may, in turn, induce sexuality to this unique liverwort in antiseptic culture. After six months of observation, under chemical treatment including GA, IAA, NAA and Vitamins and the application of red light, at 9 hour exposure daily, under 20℃, showed successful results on the initiation of sex organ on the gametophyte of Haplomitrium rotundifolium. GA 10-6M/l seemed ti induce the formation of primary archegonia consisting of few cells only, at the surface of the shoot tip. Red light at 9 hr illumination, showed the occurrence of archegonia, in mature stage on the tip of th shoot (Pl. III, 1-6). These findings, the antiseptic gametophyte completed in this laboratory in former years and initiation of sex organs in the present research are all first time reports to the biological science.