Abstract

Five-week-old plants of Zinnia elegans were grown in culture medium containing 50 M CuSO4. Plasma membrane was isolated and purified from both leaves of control and susceptible plants with method of polyethylene glycol/dextrin two-phase partition system. The major fatty acids of plasma membrane were identified as palmitic acid (16 : 0), linoleic acid (18 : 2) and linolenic acid (18 : 3). The ratios of unsaturated fatty acids to saturated fatty acids were reduced in copper-treated plants. The productions of lipid peroxides and nonprotein thiol-containing compound were obviously enhanced in copper-treated plants. Copper also caused the increment in chlorophyll content and total amounts of carotenoids in young leaves.

中文摘要

五週大的百日菊幼苗以含或不含 50 M硫酸銅的營養液培養後，以 PEG-Dextran 雙相分配系純化原生質膜的方法，收集取樣分離與測試。經分析顯示 Palmitic acid (16:0)、Linoleic acid (18 : 2) 及 Linolenic acid (18 : 3) 為三種主要的脂肪酸，不飽和脂肪酸與飽含脂肪酸的比例因銅處理而減低。銅處理也促進脂質過氧化物的生成和非蛋白質性的含硫化物的合成，同時造成百日草幼葉中葉綠素及總類胡蘿蔔素的增加。 關鍵字：銅、膜脂、色素、含硫化合物、毒害、百日草。

Keyword: Copper, Membrane lipids, Pigments, Thiol-containing compounds, Toxicity, Zinnia elegans