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Photosynthesis-related proteins of cup-shaped galls in Litsea acuminata leaves

Tin-Han Shih, Kuan-Hung Lin, Yu-Jie Chen, Szu-Hsien Lin, Chi-Ming Yang

Published on: 27 July 2020

Page: 407 - 412

DOI: 10.6165/tai.2020.65.407

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    Abstract

    Insect-induced galls are an atypical growth and differentiation form of plant tissue. The objective of this research was to study the expression of photosynthesis-associated proteins in Cecidomyiidae galls derived from the leaves of Litsea acuminata using a Western blot analysis of antibodies against light-harvesting complex (LHC) proteins isolated from non-galled and galled leaves and gall tissues. These LHC proteins involved in RC-1a, RC-1b, LHCb4, LHCb5, CP47, and CP-1a showed different responses in galls and leaves and exhibited a remarkable potential modulation role in regulating gall development. All photosynthetic proteins were repressed in gall tissues, indicating that light reaction functioning was significantly repressed. Compared to non-galled and galled leaves, galls demonstrated significantly lower chlorophyll (Chl) content and photosystem II maximum quantum efficiency (Fv/Fm) values, suggesting that insect infestations reduced photosynthetic efficiency. In addition, there were significant and positive correlations between LHCb5, CP-1a, and CP47 vs. Fv/Fm values and Chl content in galls and leaves, indicating that gall infections induced physiological changes, and therefore, that the Fv/Fm value could be utilized as a tool to easily and quickly study the eco-physiology of galls.

    Keyword: Chlorophyll fluorescence, gall, light-harvesting complex protein, photosynthesis, western blot

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