Research Paper
Morphological and molecular identification for Saxifraga yangshuoensis (sect. Irregulares, Saxifragaceae), a new species from Guangxi, China
Hai-Ling Chen, Wei-Bin Xu, Wan-Yi Zhao, Yan Liu
Published on: 13 November 2022
Page: 603 - 608
DOI: 10.6165/tai.2022.67.603
Abstract
Saxifraga yangshuoensis (Saxifragaceae), a new species from Yangshuo County in northeast Guangxi, China, is described and illustrated. It closely resembles S. damingshanensis and S. shennongii in stolons absent, leaves abaxially usually with spots, smallest petals base with yellow spotted, but differs from its leaves thickly papery, slightly revolute when dried, margin subentire, adaxially densely covered glandular hispid, abaxially densely covered with pale green spots, petiole base margin nearly glabrous and petals apex greenish. Combine morphological comparisons and phylogenetic analysis based on psbA-trnH and matK, confirmed that the new species differed from those similar species of S. sect. Irregulares.
Keyword: Chloroplast genome, Irregulares, Saxifraga damingshanensis, Saxifraga shennongii
Literature Cited
Balfour, B. 1916. Saxifrages of the Diptera Section, with description. Transactions and proceedings of the Botanical Society of Edinburgh 27(1): 65–75.
DOI: 10.1080/03746601609468463View Article
Google Scholar
Curtis, W. 1774. XXXI. A catalogue of the fifty plants, from Chelsea Garden, presented to the Royal Society, by the Worshipful Company of Apothecaries, for the Year 1773, pursuant to the direction of Sir Hans Sloane, Bart. Med. Reg. et Soc. Reg. nuper Pr?fes. Philosophical Transactions of the Royal Society of London 64(1): 302–309.
DOI: 10.1098/rstl.1774.0031View Article
Google Scholar
Darriba, D., G.-L. Taboada, R. Doallo and D. Posada 2012. "jModelTest 2: more models, new heuristics and parallel computing". Nat. Methods. 9(8): 772.
DOI: 10.1038/nmeth.2109View Article
Google Scholar
Deng, J., B.-T. Drew, E.-V. Mavrodiev, M.-A. Gitzendanner, P.-S. Soltis and E.-D. Soltis 2015. Phylogeny, divergence times, and historical biogeography of the angiosperm family Saxifragaceae. Mol. Phylogenet. Evol. 83: 86–98.
DOI: 10.1016/j.ympev.2014.11.011View Article
Google Scholar
Don, D. 1822. A monograph of the genus Saxifraga. Transactions of the Linnean Society of London 13(2): 341–452.
DOI: 10.1111/j.1095-8339.1821.tb00066.xView Article
Google Scholar
Doyle, J.-J. and J.-L. Doyle 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bulletin. 19(1): 11–15.
Edgar, R.C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32(5): 1792–1797.
DOI: 10.1093/nar/gkh340View Article
Google Scholar
Engler, A. and E. Irmscher 1913. Diagnosen neuer arten. Notizblatt des K?niglichen Botanischen Gartens und Museums zu Berlin (-Dahlem). 6: 35–37.
Gornall, R.-J., H. Ohba and J.-T. Pan 2000. New taxa, names, and combinations in Saxifraga (Saxifragaceae) for the Flora of China. Novon. 10(4): 375–377.
DOI: 10.2307/3392990View Article
Google Scholar
Hall, T.-A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analyses program for Windows 95/98/NT. Nucleic Acids Symposium Series. 41: 95–98.
Haworth, A.-H. 1803. Miscellanea naturalia: sive dissertationes vari? ad historiam naturalem spectantes. Typis J. Taylor, Londini, 155 pp.
IUCN 2012. IUCN Red List Categories and Criteria: Version 3.1. Second edition. IUCN, Gland, Switzerland and Cambridge, iv + 32 pp.
Linnaeus, C. 1753. Species plantarum. Laurentius Salvius, Stockholm, pp. 398–405.
Pan, J.-T. 1991. A Study on the Genus Saxifraga L. from China. Acta Phytotaxon. Sin. 29: 1–24.
Ronquist, F., M. Teslenko, P. van der Mark , D.-L. Ayres, A. Darling, S. H?hna, B. Larget, L. Liu, M.-A. Suchard and J.-P. Huelsenbeck 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61(3): 539–542.
DOI: 10.1093/sysbio/sys029View Article
Google Scholar
Soltis, D.-E., L.-A. Johnson and C. Looney 1996. Discordance between ITS and chloroplast topologies in the Boykinia group (Saxifragaceae). Syst. Bot. 21(2): 169–185.
DOI: 10.2307/2419746View Article
Google Scholar
Soltis, D.-E., R.-K. Kuzoff, M.-E. Mort, M. Zanis, M. Fishbein, L. Hufford, J. Koontz and M.-K. Arroyo 2001. Elucidating deeplevel phylogenetic relationships in Saxifragaceae using sequences for six chloroplastic and nuclear DNA regions. Ann. Mo Bot. Gard. 88 (4): 669–693.
DOI: 10.2307/3298639View Article
Google Scholar
Soltis, D.-E., M.-M. Mort, M. Latvis, E.-V. Mavrodiev, B.-C. O’Meara, P.-S. Soltis, J.-G. Burleigh and R. Rubio De Casas 2013. Phylogenetic relationships and character evolution analysis of Saxifragales using a supermatrix approach. Am. J. Bot. 100(5): 916–929.
DOI: 10.3732/ajb.1300044View Article
Google Scholar
Stamatakis, A., P. Hoover and J. Rougemont 2008. A rapid bootstrap algorithm for the RAxML web-servers. Syst. Biol. 57(5): 758–771.
DOI: 10.1080/10635150802429642View Article
Google Scholar
Tate, J.-A. and B.-B. Simpson 2003. Paraphyly of Tarasa (Malvaceae) and diverse origins of the polyploidy species. Syst. Bot. 28(4): 723–737.
Tkach, N., M. R?ser, G. Miehe, A.-N. Muellner-Riehl, J. Ebersbach, A. Favre and M.-H. Hoffmann 2015. Molecular phylogenetics, morphology and a revised classification of the complex genus Saxifraga (Saxifragaceae). Taxon. 64(6): 1159–1187.
DOI: 10.12705/646.4View Article
Google Scholar
Wang, G.-F., Y.-S. Ye and F.-W. Xing 2008. Saxifraga daqiaoensis (Saxifragaceae), a new species from Guangdong, China. Ann. Bot. Fenn. 45(3): 237–239.
DOI: 10.5735/085.045.0313View Article
Google Scholar
Xiang, C.-L., M.-A. Gitzendanner, D.-E. Soltis, H. Peng and L.-G. Lei 2012. Phylogenetic placement of the enigmatic and critically endangered genus Saniculiphyllum (Saxifragaceae) inferred from combined analysis of plastid and nuclear DNA sequences. Mol. Phylogenet. Evol. 64(2): 357–367.
DOI: 10.1016/j.ympev.2012.04.010View Article
Google Scholar
Zhang, D., F.-L. Gao, I. Jakovli?, H. Zou, J. Zhang, W.-X. Li and G.-T. Wang 2020a. PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Mol. Ecol. Resour. 20(1): 348–355.
DOI: 10.1111/1755-0998.13096View Article
Google Scholar
Zhang, J.-J., W.-Y. Zhao, K.-K. Meng, W.-B. Liao, L. Wang and S.-F. Chen 2019. Saxifraga shennongii, a new species of Saxifragaceae from Hunan Province, China. Phytotaxa. 418(1): 79–88.
DOI: 10.11646/phytotaxa.418.1.4View Article
Google Scholar
Zhang, M.-H., X.-S. Zhang, Z.-L. Nie, D.-G. Zhang and Y. Meng 2017. Saxifraga kegangii (Saxifragaceae), a new species from Hunan Province of central China. Phytotaxa. 309(2): 159–165.
DOI: 10.11646/phytotaxa.309.2.7View Article
Google Scholar
Zhang, M.-H., C.-Y. Wang, C. Zhang, D.-G. Zhang, K.-G. Li, Z.-L. Nie and Y. Meng 2020. Phylogenetic relationships and biogeographic history of the unique Saxifraga sect. Irregulares (Saxifragaceae) from eastern Asia. J. Syst. Evol. 58(6): 958–971.
DOI: 10.1111/jse.12547View Article
Google Scholar
Zhang, X.-J., Q.-S. Fu, J.-T. Chen, L.-J. Li, P.-R. Luo, J.-Y. Peng, X.-H. Huang, H. Sun and T. Deng 2021. Saxifraga viridiflora (Saxifragaceae), an unusual new species from Guangxi, China. PhytoKeys. 184: 19–26.
DOI: 10.3897/phytokeys.184.73421View Article
Google Scholar
Zhang, X.-J., T.-H. Kuang, J.-T. Chen, L.-J. Li, J.-Y. Peng, K.-L. Guan, X.-H. Huang, H.-C. Wang and T. Deng 2022. Saxifraga sunhangiana (Saxifragaceae), a new species from Gansu, China. Taiwania. 67(2): 195–200.
DOI: 10.6165/tai.2022.67.195View Article
Google Scholar
Zhang, X.-J., Z.-C. Liu, K.-K. Meng, Q.-L. Ding, L. Wang and W.-B. Liao 2018. Saxifraga luoxiaoensis (Saxifragaceae), a new species from Hunnan and Jaingxi, China. Phytotaxa. 350(3): 291–296.
DOI: 10.11646/phytotaxa.350.3.8View Article
Google Scholar
Zhao, W.-Y., K.-K. Meng, Q. Fan, J.-H. Jin and W.-B. Liao 2019. Saxifraga damingshanensis (S. sect. Irregulares, Saxifragaceae), a new species from Guangxi, China. Phytokeys. 133: 95–103.
DOI: 10.3897/phytokeys.133.36704View Article
Google Scholar