Research Paper

Morphological and molecular evaluation of Japanese Plicanthus R.M.Schust. (Anastrophyllaceae, Marchantiophyta)

Kyousuke Amamoto, Yuya Inoue, Mu Mu Aung, Tomio Yamaguchi

Published on: 01 June 2023

Page: 250 - 254

DOI: 10.6165/tai.2023.68.250


Plicanthus birmensis and P. hirtellus have been recognized in Japan. These species could be distinguished by features of ciliate leaf lobes and underleaves, and anatomy of stems. However, intermediate forms between these species can be found in Japan, and the circumscriptions of these species are unclear among Japanese populations. We evaluate morphological and molecular variations in Japanese Plicanthus to address the taxonomic identity of Japanese populations. The Japanese samples identified as P. birmensis, P. hirtellus and intermediate forms between them were nested within a clade. Among Japanese populations no significant correlation between morphological characters and phylogenetic relationships is confirmed, and the diagnostic characters to delimit these two species in Japan should be recognized as infraspecific variation. Thus, we recognize only one species, Plicanthus hirtellus in Japan.

Keyword: Anastrophyllaceae, Plicanthus hirtellus, Plicanthus birmensis, phylogenetic relationships, infraspecific variation

Literature Cited

Ando, H., Seki, T., Kanda, H., Deguchi, H., Matsumoto, K. 1975 Bryophytes of Itsukushima (Miyajima) Island Southwestern Japan. In Committee for the Urgent Investigation of the Primeval Forest and Scenic Reserves of Itsukushima (Miyajima) Island (ed.), Land and Life in Itsukushima (Scientific Studies of Itsukushima Island, Southwestern Japan): 333??76 + Pl. 44??5. Miyajima, Hiroshima, Japan.

Bakalin, V., Vilnet, A., Nguyen, V.S. 2020 Vietnamiella epiphytica ??a new genus and species of Anastrophyllaceae (Hepaticae). Bryologist 123(1): 48??3.
DOI: 10.1639/0007-2745-123.1.048View Article Google Scholar

Grolle, R. 1995 The Hepaticae and Anthocerotae of the East African Islands. An Annotated Catalogue. Bryophytorum Bibliotheca 48: 1??78.

Hattori, S. 1966 Anthocerotae and Hepaticae. The flora of eastern Himalaya: 501??36.

Inoue, Y., Tsubota, H., Kubo, H., Uchida, S., Mukai, S., Shimamura, M., Deguchi, H. 2011 A note on Pottia intermedia (Turner) F?rnr. (Pottiaceae, Bryopsida) with special reference to its phylogeny and new localities in SW Japan. Hikobia 16: 67??8. (see Supplement)

Inoue, Y., Tsubota, H., Sato, H., Yamaguchi, T. 2012 Phylogenetic note on Pachyneuropsis miyagii T.Yamag. (Pottiaceae, Bryophyta). Hikobia 16: 221??28. (see Supplement)

Inoue Y., Tsubota, H. 2014 On the systematic position of the genus Timmiella (Dicranidae, Bryopsida) and its allied genera, with the description of a new family Timmiellaceae. Phytotaxa 181(3): 151??62.
DOI: 10.11646/phytotaxa.181.3.3View Article Google Scholar

Kis, G., P?cs, T. 1997 Oil body studies on African hepaticae. Journal of the Hattori Botanical Laboratory 81: 175??42.

Katagiri, T., Furuki, T. 2018 Checklist of Japanese liverworts and hornworts, 2018. Hattoria 9: 53??02.

Katagiri, T., Inoue, Y. 2018 Phylogenetic position of the East Asian endemic genus Hattoria (Anastrophyllaceae) based on chloroplast DNA sequence. Hattoria 9: 41??2.

Katoh, K., Standley, D.M. 2013 MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol. Biol. Evol. 30(4): 772??80.
DOI: 10.1093/molbev/mst010View Article Google Scholar

Kitagawa, N. 1965 A revision of the family Lophoziaceae of Japan and its adjacent regions. I. J. Hattori Bot. Lab. 28: 239??91.

Kodama, T. 1971 Liverworts of Kinki District, Central Japan, preserved in the Osaka Museum of Natural History, Part 1: Introduction, Calobryales, and Jungermanniales (1). Osaka Museum of Natural History, Osaka, Japan. 116 pp.

Kumar, S, Stecher, G., Tamura, K. 2016 MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol. Biol. Evol. 33(7): 1870??874.
DOI: 10.1093/molbev/msw054View Article Google Scholar

Masuzaki, H., Shimamura, M., Furuki, T., Tsubota, H., Yamaguchi, T., Majid, A., Deguchi, H. 2010 Systematic position of the enigmatic liverwort Mizutania (Mizutaniaceae, Marchantiophyta) inferred from molecular phylogenetic analyses. Taxon 59(2): 448??58. (see Supplement)
DOI: 10.1002/tax.592010View Article Google Scholar

Oguri, E., Yamaguchi, T., Tsubota, H., Deguchi, H. 2003 A preliminary phylogenetic study of the genus Leucobryum (Leucobryaceae, Musci) in Asia and the Pacific based on ITS and rbcL sequences. Hikobia 14(1): 45??3. (see Supplement)

Rambaut, A., Drummond, A.J., Xie, D., Baele, G., Suchard, M.A. 2018 Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Syst. Biol. 67(5): 901??04.
DOI: 10.1093/sysbio/syy032View Article Google Scholar

Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., H?hna, S., Larget, B., Liu, L., Suchard, M.A., Huelsenbeck, J.P. 2012 MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61(3): 539??42.
DOI: 10.1093/sysbio/sys029View Article Google Scholar

Schuster, R.M. 2002a Austral Hepaticae Part II. Nova Hedwig. Beih. 119: 1??06.

Schuster, R.M. 2002b Revisionary studies of Chandonanthoideae. Nova Hedwigia 74(3??): 465??96.
DOI: 10.1127/0029-5035/2002/0074-0465View Article Google Scholar

Schwarz, G. 1978 Estimating the dimension of a model. Ann. Stat. 6: 461??64.
DOI: 10.1214/aos/1176344136View Article Google Scholar

S?derstr?m, L., Hagborg, A., Konrat, M., Bartholomew-Began, S., Bell, D., Briscoe, L., Brown, E., Cargill, D.C., Costa, D.P., Crandall-Stotler, B.J., Cooper, E.D., Dauphin, G., Engel, J.J., Feldberg, K., Glenny, D., Gradstein, S.R., He, X., Heinrichs, J., Hentschel, J., Ilkiu-Borges, A.L., Katagiri, T., Konstantinova, N.A., Larra?n, J., Long, D.G., Nebel, M., P?cs, T., Puche, F., Reiner-Drehwald, E., Renner, M.A.M., Sass-Gyarmati, A.A., Sch?fer-Verwimp, J.G., Moragues, S., Stotler, R.E., Sukkharak, P., Thiers, B.M., Uribe, J., V??a, J., Villarreal, J.C., Wigginton, M., Zhang, L., Zhu, R. 2016 World checklist of hornworts and liverworts. PhytoKeys 59: 1??28.
DOI: 10.3897/phytokeys.59.6261View Article Google Scholar

Souza-Chies, T.T., Bittar, G., Nadot, S., Carter, L., Besin, E., Lejeune, B. 1997 Phylogenetic analysis of Iridaceae with parsimony and distance methods using the plastid gene rps4. Plant Syst. Evol. 204(1-2): 109??23. (see Supplement)
DOI: 10.1007/BF00982535View Article Google Scholar

Stamatakis, A. 2014 RAxML Version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30(9): 1312??313.
DOI: 10.1093/bioinformatics/btu033View Article Google Scholar

Sugiura, N. 1978 Further analysis of the data by Akaike? information criterion and the finite corrections. Commun. Stat. - Theory Methods 7(1): 13??6.
DOI: 10.1080/03610927808827599View Article Google Scholar

Suzuki, T., Inoue, Y., Tsubota, H., Iwatsuki, Z. 2013 Notes on Aptychella (Sematophyllaceae, Bryopsida): Yakushimabryum longissimum, syn. nov. Hattoria 4: 107??18.

Taberlet, P., Gielly, L., Pautou, G., Bouvet, J. 1991 Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol. 17(5): 1105??109. (see Supplement)
DOI: 10.1007/BF00037152View Article Google Scholar

Tanabe, A.S. 2011 Kakusan4 and Aminosan: two programs for comparing nonpartitioned, proportional and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Mol. Ecol. Resour. 11(5): 914??21.
DOI: 10.1111/j.1755-0998.2011.03021.xView Article Google Scholar

Tsubota, H., Nakao, N., Arikawa, T., Yamaguchi, T., Higuchi, M., Deguchi, H., Seki, T. 1999 A preliminary phylogeny of Hypnales (Musci) as inferred from chloroplast rbcL sequence data. Hikobia 7: 233??48. (see Supplement)

Tsubota, H., Ikeda, S., Inoue, Y., Uchida, S., Mukai, S. 2015 Current status of Lysimachia sikokiana Miq. (Primulaceae) in Hiroshima Prefecture, SW Japan. Hikobia 17: 81??8. (see Supplement)

Udar, R., Kumar, A. 1982 Studies in east Himalayan hepaticae-II: The genus Chandonanthus Mitten. Geophytology 12(1): 59??4.